| Object:To investigate the intra-retinal expression of neuroglobin (Ngb) and death of retinal ganglion cells (RGCs) in acute retina ischemia rats.Methods:It was an experimental study. There were all 40 female Sprague-Dawley rats which were 2-3 moon's ages and 220-240 g weight. The acute retina ischemia model was established by specific hypodesised left retina artery of Sprague-Dawdey rats. Forty rats were divided into four groups (0,15,30,60min) by the time of retina ischemia. Every group has 10 rats, in one group random 3 rats were detected by Western blotting; 4 rats were detected by retrogradely labeled RGCs counted with a fluorogold dye (FG) and immunohistochemisty fluorescence intensity analysis; The rest 3 rats were detected by Western blotting. The difference among different data were analyzed statistically by One-factor analysis of variance and LSD-t analysis。Results:The intra-retinal expression of Ngb reached maxlmum after acute ischemia 15 minute (P=0.000), then the expression began decreasing. After 30 minute acute ischemia, the expression of Ngb had approached normal (P=0.728) while, the cell number of RGCs began lower than 0 min group (P=0.011); after 60 minute acute ischemia, the expression of Ngb had been obviously lower than 0 min group (P=0.001), the cell number of RGCs had been further lower than 0 min group (P=0.000); The expression of Ngb in RGCs layer was highest in rat retina. The expression in inner plexiform layer and external plexiform layer were lower than the former. The expression of Ngb RGCs was mostly intracytoplasm. After 30 minute acute ischemia, the expression of Ngb were detected in mitochondrial outer compartment and mitochondrial cristae, but in cytoplasm of inner nuclear layer and outer nuclear layer the Ngb was not found.Conclusion Ngb quickly steps-up when RGCs die in acute retina ischemia, and mainly expresses intracytoplasm of RGCs. It has tense relationships with nerve cells' survival in hypoxia. |
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