Effects Of Fei-Xian-Ling â…¡ On Pulmonary Fibrosis With Animal Model Induced By Bleomycin And Its Mechanisms

Objective:To investigate the effects of Fei-Xian-LingⅡon pulmonary fibrosis with animal model induced by bleomycin and its mechanisms.Methods: Kunming strian mice and Sprague-Dawley rats were randomly divided into control group,model group,prednisolone group and FXL-Ⅱhigh-dose,middle- dose,low-dose group.Models were reproduced by intratracheal injection of bleomycin. On the second day,the animals in each curing group were orally administrated corresponding dose of FXL-Ⅱ,control group and model group were orally administra -ted corresponding dose of CMC-Na. After continully administrated of 28 d, models in each group were sacrificed,and lungs were havested for investigating of pathomorphological changes by HE staining,Masson staining and electron microscope.The extent of plumonary alveolitis and fibrosis were evaluated by Szaopiel's method; the level of SOD,MDA,T-AOC,NOS,SDH,OH-·,HYP in serum and lung tissue were determinated by biochemical analysis,expression of TGF-β1,Smad2/3, Col-Ⅲ,NF-κB,α-SMA, Cav-1,OPN protein in lung tissue were detected by immunohistochemical methods.Results: (1)FXL-Ⅱcould inhibit body weight loss in the animal models induced by bleomycin and reduce lung index in some extent;(2) FXL-Ⅱcould decrease the extent of alveolitis, fibrosis and ultrastructural injury(P<0.05,P<0.01);(3) FXL-Ⅱcould obviously down-regulate the expression of TGF-β1,Smad2/3,NF-κB,Col-Ⅲ,α-SMA,OPN protein in lung tissue(P<0.05,P<0.01),but up-regulate the expression of Caveolin-1;(4) FXL-Ⅱcould dramatically decrease the content of HYP in the lung tissue(P<0.05,P<0.01, P<0.001);(5) Biochemical indicator results displayed that FXL-Ⅱcould significantly enhance the activity of SOD and SDH(P<0.05,P<0.01, P<0.001),reduce the content of MDA(P<0.05,P<0.01), improve the capacity of T-AOC and inhibiting hydroxy radical(P<0.05,P<0.01), attenuate the activity of NOS(P<0.05,P<0.01, P<0.001).Conclusion:FXL-Ⅱmight have some disturbing effects on pulmonary fibrosis induced by bleomycin,and the mechanisms might be associated with its effects of anti-inflammatory, anti-oxidation, scavenging free radical, mitochondrial protection, anti-fibroblastic proliferation, inhibiting TGF-β/ Smads signaling pathway, inhibiting depositing of Col-Ⅲin lung tissue,down-regulating the expression of NF-κB, Col-Ⅲ,α-SMA,OPN protein and up- regulating the expression of Caveolin-1 protein in lung tissue.