| Objective:To explore effect of intra-articular injection of recombinant human acidic fibroblast growth factors on the expression of matrix metal proteinases-1 in the cartilage and synovium of early traumatic arthritis model in male SD rats. Methods: 24 Male SD rats were randomly divided into blank groups (n=8), control group(n= 8) and experimental group (n = 8), The animal models of TA were developed through transection of the medial collateral ligament plus partial medial meni-scectomy in male SD rats. 1μg rh-aFGF was injected into articular cavity during and one week after operation. Two weeks after the operation, rats were sacrificed, HE staining was applied to observe the changes of the structure of cartilage and synovium, and immunohistochemical staining was applied to detect the expression of MMP-1 in cartilage and synovium. Results: The expression of MMP-1 in cartilage and synovium in the control group rats increased significantly as compared with the blank group rats. While the expression of MMP1 in articular cartilage and synovial membrane of experimental group rats is abated. Conclusion: Intra-articular injection of rh-aFGF can reduce the expression of MMP1 in articular cartilage and synovial tissue of early traumatic arthritis rats, and can inhibit the degeneration of the articular cartilage and inflammatory of the synovial tissues to a certain extent. |
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