Studying On The Chemical Constituents Of Three Dai Medicines

Microcos paniculatal L. belongs to Tiliaceae genus of Microcos L.,located in Guangdong,Guangxi,Hainan,Yunnan,Indochina Peninsula,India and other places. Their leaves can be used as medicine,for heat dampness,and be health for stomach. It also be treated for cold and fever,jaundice,loss of appetite,indigestion,abdominal pain,abdominal distention,diarrhea,sore,centipede Song bites and so on.Glycosmis pentaphylla belongs to Rutaceae genus of Glycosmis Linn plant.Most plants of Glycosmis Linn are shrub or small tree. There are more than 50 species distributed in southern southeastern Asia,and northeastern Australia. There are 11 species and 1 mutation. Glycosmis Linn contains a variety chemical composition and mainly containing carbazole,acridone,quinolone and quinazoline alkaloids,amides of sulfur compounds,terpenoids,and isoflavones which have been discovered containing celery sugar in recent years from Glycosmis Linn. Alkaloids as its major bioactive components , has various biological activities such as anti-tumor ,anti-bacterial etc. This plant is mainly distributed in Fujian,Guangdong,Guangxi,Yunnan and other places. To the root and leaves used as medicine,Glycosmis pentaphylla,also known as "mountain of orange leaves",is commonly used herbs with its root and leaves,with the medical treatment of stasis,phlegm elimination product,Qufeng detoxification.Fissistigma polyanthum,also known as Black wind vine,ventilation Hong,Dali Wan,niu gales and so on,belonges to Annonaceae genus of Fissistigma plant,which can be discovered in the valley,river,street and damp places. It has the medical effect of through meridian and strengthening the bones and spleen .In civil,it is used for bruises,rheumatoid arthritis,rheumatoid joint pain,cold and irregular menstruation embolism. We also found that Fissistigma polyanthumcream has the biological activity of antivirus by pharmacological study.In this paper,by using the system solvent extraction,normal phase and reversed phase silica gel column chromatography,HPLC and other modern separation technology,respectively,12 compounds were isolated from three kinds of three Dai medicine extracts. Six compounds were isolated from Microcos paniculatal L,including one flavonoid,a steroid compound,a carbohydrate compound,three terpenoids and four. Four compounds were isolated from Glycosmis pentaphylla,including three carbazole compounds and a amide compound. Two compounds were isolated from Fissistigma polyanthum,including one chalcone compound and one nor-sesquiterpenoid compound were identified by using modern spectroscopic techniques. Among these 12 compounds isolated from three plants for the first time,there is a new one,3β-O-p-hydroxy-E-cinnamoyloxy-2α,23-dihyd-roxy-olean-12-en- 28-oate(5).The other 11 known compounds are called epicatechin(1) ,Sucrose(2) ,β-sitosterol(3),3-trans-feruloyl maslinic acid(4),maslinic acid(6),glybomine B(7),glycoborinine(8),carbalexin A(9),N-p-coumaroyltyramine(10),kanakugiol(11),teutenone A(12). These 11compounds were isolated from these plants for the first time.We use DPPH oxidation to measure the antioxidant activity of the 40 Dai extracts in 9 drugs .The results show that scavenging capacity of DPPH of the Fissistigma polyanthum and the Microcos paniculatal L. is higher than that of Synthetic antioxidants (IC50 = 20.0μM) .It has strong antioxidant activity...