Meta-analysis On The Association Between The Polymorphism Of VDR,NRAMP1 And IL-10 And Tuberculosis Susceptibility

Objectives To provide a quantitative summary in estimating the association between polymorphisms of vitamin D receptor gene, NRAMP1 gene, IL-10 gene and susceptibility to tuberculosis by means of meta-analysis.Methods All of the articls were searched using"VDR","NRAMP1","IL-10","polymorphisms"or"alleles", in combination with"tuberculosis"on Databases CNKI, CBM, WanFang DATA, Medline and Pubmed. They were original case-control studies on the relationship between Variants of the natural resistance-associated macrophage protein 1, vitamin D receptor, Interleukin-10 and tuberculosis susceptibility, and published between January 1980 and November 2007. The articls with poor-qualified studies were excluded. RevMan4.2 was applied in the process of analysing these data of 14,16,and 6 case-control studies. The Peto odds ratios were calculated by applying fixed effect model if heterogeneity did not exist among the results but the odds ratios were calculated by applying Dersimonian-Laird random effect model in case of heterogeneity. Potential publication bias were assessed by using a funnel plot. And the fail-safe number is applied to estimate publication bias quantitatily.Results There was no significant heterogeneity in 14 studies on the relationship between the VDR gene and tuberculosis susceptibility. The summary ORs for 14 studies with polymorphisms of the VDR gene were1.12(95%CI:0.98-1.28) in Ff vs FF,1.66(95%CI:1.12-2.46) in ff vs FF,0.97(95%CI:0.82-1.14) in Tt vs TT,1.27(95%CI:0.96-1.69) in tt vs TT. The case-control study consistency in 16 articles on the relationship between the NRAMP1 gene and tuberculosis susceptibility was fairly identified. The summary ORs for 16 studies with polymorphisms of the NRAMP1 gene were 1.50(95%CI:1.29-1.75) in TGTG+- vs TGTG++, 0.89(95%CI:0.59-1.34) in TGTG-- vs TGTG++,1.39(95%CI:1.20-1.61) in GA vs GG,1.07(95%CI:0.69-1.67) in AA vs GG, 1.30(95%CI:1.09-1.56) in GC vs GG, 1.64(95%CI:0.90-3.00)in CC vs GG. The case-control study consistency in 6 articles on the relationship between the IL-10 gene and tuberculosis susceptibility was fairly good. The summary ORs for 6 studies with polymorphisms of the IL-10 gene were 0.89(95%CI:0.73-1.09) in AG vs AA, 1.14(95%CI:0.74-1.75) in GG vs AA. No publication bias were found by using a funnel plot. And conclusion is stable by sensibility analyses.Conclusion Polymorphisms at FokI loci (VDR-ff), 3'UTR of NRAMP1(TGTG+-) and D543N of NRAMP1(GA) have statistically significant association between the VDR and NRAMP1 variants and susceptibility to tuberculosis, and variant in the other locus failed to show statistically significant association.