Protection Effects Of Grape-seed Proanthocyanidins Extrac On Renal Ischemia Reperfusion Injury In Rats

Objective:To investigate the Anti-oxidation Effects of grape-seed proanthocyanidins extrac(GSPE) on Renal Ischemia Reperfusion Injury in Rats.Methods:Thirty-six Wistar rats weighting(180-220g)were divided into three groups randomly:sham operation group(Sham group),renal ischemia reperfusiona injury group(RIRI group), grape seed proanthocyanidins group(GSPE group).There were 6 rats in each group.The content variation of serum creatinine(Scr),blood urea nitrogen(BUN),nitric oxide(NO) in plasma and the activity variation of inducible nitric oxide synthase(iNOS) in plasma,the content variation of MDA in plasma and in tissue and the activity variation of GSH-Px in plasma and in tissue,the intercellular adhesion molecules-1(ICAM-1)expression were detected respectively in renal ischemia-reperfusion 2h,24h as well as pathological changes in the different time were also noted by light microscope. Results:Compared with the Sham group,the contents of Scr,BUN,NO and iNOS in plasma and MDA in plasma and in tissue significantly were higer in RIRI group and in GSPE group(p<0.05),and activity of GSH-Px was lower in RIRI group and in GSPE group(p<0.05); Compared with the RIRI group, the contents of Scr,BUN,NO and iNOS were lower in GSPE group,the contents of MDA in plasma and in tissue were also lower (p<0.05);and the activity of GSH-Px were step up (p<0.01);expression of ICAM-1 in rat kidneys in the GSPE group was significantly decreased than that of the RIRI group(p<0.05).The renal pathological changes were more severe in RIRI group than that of GSPE group,and GSPE2h group had no significant difference compared with GSPE24h.Conclusion:The GSPE played a protective role on the kidney in the process of renal ischemia reperfusion injury,the mechanism may be related to anti-free radical injury and reducing the lipid peroxidation.