Intervention Effect Of Grape Seed Proanthocyanidins On Acute Renal Injury In Mice

Acute kidney injury(AKI)is a clinical syndrome induced by several factors,which characterized by renal dysfunction,renal tubular and glomerular lesions,oliguresis and anuresis.In clinical,glucocorticoid dexamethasone(Dex)is the preferred drug for the treatment of AKI,but has the side effects of increasing body weight,inducing osteoporosis and others.However,there is no effective drugs to prevent or treat AKI currently.Grape Seed Extract Procyanidins(GSPE)is a naturally derived polyphenol biologically flavonoid with renal protective function.In order to study the protective effect of GSPE and compare with the Dex on AKI mice,mice were used as experimental subjects.10021-day-old kunming mice were randomly divided into five groups which remain 20 rats in each group:blank control group(C group),GSPE treatment group(P group),LPS treatment group(L group),GSPE and LPS treatment group(PL group),Dex and LPS treatment group(DL group),20 rats in each group.Mice in P group and PL group were injected with GSPE(5 mg/kg B.W),C group and L group were injected with 0.9%NaCl(5 mL/kg B.W.)and DL group was injected with(3 mg/kg B.W.)in caudal vein.One hour after GSPE treatment,L group,PL group and DL group were injected intraperitoneally with LPS(10 mg/kg B.W.).C group and P group were injected intraperitoneally with 0.9%NaCl(5 mL/kg B.W.).The urine volume of 24 h from LPS injection was gathered then was measured,the body weight of mice was weighed,and the blood was collected from eyeball.And then the mice were dissected,the kidneys were collected and were weighed to observe the renal pathological changes and renal function(UA,Cre,Urea,CHO and BUN),antioxidant capacity(GSH-Px,T-SOD and CuZn-SOD,CAT),inflammation related factors(NO,iNOS,TNF-?,TLR4 and NF-?B).The results show:(1)Compared with C group,L group were mental fatigue,oliguresis,renal dysfunction andrenal structural damage.Compared with L group,the renal structure damage of PL group and DL group was decreased,the urinary output was normal,and the serum BUN and Cre were significantly decreased(p<0.001),which indicated that GSPE protects LPS-induced mouse kidney structure and function damage,and has the similar effect of Dex.(2)Compared with C group,the activities of CAT,T-SOD and CuZn-SOD in renal tissue of L group were significantly decreased(p<0.01;p<0.001),and the content of MDA was significantly increased(p<0.001).Compared with L group,the activities of CAT,T-SOD and CuZn-SOD of PL group and DL group were significantly increased(p<0.05;p<0.001),MDA content was significantly decreased(p<0.001).GSPE has the similar effect with Dex on resistance of LPS-induced oxidative stress in mice.(3)Compared with C group,the levels of NO,NOS,IL-6 and the gene expression levels of IL-1,IL-1?,TNF-a,TLR4 and NF-?B were significantly increased in L group(p<0.05;p<0.001).Compared with L group,these indexes except iNOS were significantly decreased in PL group and DL group(p<0.01;p<0.001),which indicated that GSPE could resist LPS-induced renal inflammatory response and have the similar effect of Dex.In summary,GSPE can effectively relieve/resist LPS-induced AKI mice urine output reduction,renal structural and functional damage,renal oxidative stress and inflammatory response,and its effect is similar to Dex.