| Background and objective The regeneration of Central nervous system (CNS) after the injury is the important and difficult point of rehabilitation research. Until now, no treatment has shown efficacy. Myelin associated inhibitory factors(MAIFs) such as MAG,NogoA and OMgp have been proved to play a key role in inhibition of axonal sprouting and regeneration after injury.These factors can interact with Nogo receptor complex and inhibite neurite outgrowth. LINGO1 is a component of the NgR1 receptor complex involved in RhoA activation. It can interact with TrkA and regulate oligodendrocyte differentiation and myelination. It can interact with EGFR and regulate neuron survival.So,it has an important role in CNS repair.Electrical stimulation can improve motor recovery in patients with stroke, but the mechanisms remain unknown. The main goal of this study is to study the expressions of LINGO1 and NgR1 at different time points after cerebral ischemia and the influence of electrical stimulation.Methods 200 Spragne-Dawley(SD)rats were subjected to establish the focal cerebral infarction model and randomly divieded into normal group, sham operation group,ischemic group and electrical stimulation group. The animal model of cerebral ischemia was established by middle cerebral artery occlusion(MCAO) method.In electrical stimulation group ,the intervention was used at 90mins after modeling.We selected"renzhong","baihui"as stimulating points for treating 30mins,once a day.The expressions of LINGO1 and NgR1 were measured with immunohistochemistry and RT-PCR at the first day,the third day,the 7th day,the 14th day and the 21st day respectively after the ischemia.The motor recovery of SD rats after cerebral infarction was studied by Longa scores.Resultsâ‘ The expressions of LINGO1 and NgR1 in the ischemic cortex increased significantly. The expressions of LINGO1 mRNA and protein in ischemic group reached the peak at the first day.The expressions of NgR1 mRNA and protein in ischemic group reached the peak at the third day.â‘¡Motor function significantly recovered in electrical stimulation group compared with the ischemic group(P<0.05).â‘¢The expression of LINGO1 protein decreased in electrical stimulation group at the 7th day (P<0.05). The expressions of NgR1 mRNA and NgR1 protein decreased in electrical stimulation group at the third day and the 7th day(P<0.05). The expressions of LINGO1 mRNA and protein in electrical stimulation group are higher than ischemic group at the 21st day (P<0.01).Conclusionâ‘ The results suggest that LINGO1 and NgR1 are involved in the pathophysiologic procedures of cerebral ischemia. The expressions of LINGO1 mRNA and protein in ischemic group reached the peak at the first day(P<0.01).The expressions of NgR1 mRNA and protein in ischemic group reached the peak at the third day (P<0.01).â‘¡Electrical stimulation can improve motor recovery after acute cerebral infarction.â‘¢The decreased expressions of LINGO1 protein, NgR1 mRNA and NgR1 protein induced by electrical stimulation may be one of the mechanisms of motor recovery after acute cerebral infarction.â‘£The increased expression of LINGO1 in electrical stimulation group at the 21st day may be response to neuronal activity. |
PDF Full Text Request