Research On The Relationship Of Vaspin And The Role In Treating Type 2 Diabetic Rats With RYGB Surgery

Objective: To explore the possible mechanism of Roux-en-Y gastric bypass (RYGB) on type 2 diabetes mellitus and provide theoretical basis for the wide clinical application of RYGB. We observe the effect of RYGB in treating T2DM rats and the changes of vaspin post operation. At the same time, we investigated the correlation between vaspin and T2DM.Methods: (1) Six-weeks-age male SD rats fed high-fat eight weeks, given STZ(30mg/kg) intraperitoneal injection, and setup in type 2 diabetic rats model. (2) Twenty type 2 diabetic rats were randomly divided into two groups: type 2 diabetes RYGB operation group(DRO group), type 2 diabetes-sham group(DSO group), ten in each group. On the same time, twenty weeks and sex-matched non-manufacturing modules SD rats were randomly divided into two groups: normal RYGB operation group(NRO group),normal-sham operation group(NSO group),ten in each group. DRO group and NRO group rats were underwent by RYGB surgery, but DSO group and NSO group rats were underwent by sham operation. (3) Body weight ,fasting plasma glucose (FPG), plasma insulin(INS) level and vaspin by ELISA kit were determined before surgery and four and eight weeks after surgery. (4) Rats adipose tissue was harvested eight weeks postoperatively and analyzed by using reverse transcription polymerase chain reaction (RT-PCR) to detect the vaspin mRNA expression.Results:1 The comparison of pre-and postoperative weight The postoperative fasting weight in DRO group rats declined gradually, but pre- and post- operative changes in the weight was not statistically significant(P>0.05).The postoperative fasting weight rose continuously in DSO group rats, NRO group and NSO group, and the weight of following surgery was significantly higher than the postoperative, which was statistically significant differences(P<0.05).2 The comparison of pre- and postoperative FPGCompared with the preoperation, four weeks, FPG in DRO group rats decreased, and there was statistically significant eight weeks postoperatively(P<0.05). DSO group, NRO group and NSO group had no significant changes postoperatively(P>0.05).3 The comparison of pre- and postoperative vaspinCompared with the preoperation, fasting vaspin gradually reduced in DRO group rats, but there was not statistically significant four-week postoperatively(P>0.05), fasting vaspin in DRO group rats further reduced obviously with statistical significance eight-week postoperatively(P<0.05). DSO group, NRO group and NSO group had no significant changes postoperatively(P>0.05).4 The comparison of pre- and postoperative INSCompared with the preoperation, fasting INS in DRO group rats gradually reduced, but there was not statistically significant four-week postoperatively(P>0.05), fasting INS of DRO group further reduced obviously with statistical significance eight-week postoperatively(P<0.05). DSO group, NRO group and NSO group had no significant changes postoperatively(P>0.05). 5 The comparison of pre- and postoperative HOMA-IRCompared to before surgery, HOMA-IR decreased in DRO group rats, and there was statistically significant four weeks and eight weeks postoperatively(P<0.05). DSO group, NRO group and NSO group had no significant changes postoperatively(P>0.05).6 The comparison of vaspin mRNA expression in rats adipose tissue eight weeks after surgeryEight weeks after surgery, vaspin mRNA expression in DSO group rats adipose tissue was higher than those in DRO group rats, NRO group rats and NSO group rats, which was statistically significant(P<0.05), there is no significant difference between DRO group,NRO group,and NSO group(P>0.05).7 The level of before and after surgery serum vaspin and FBG, INS, HOMA-IR correlation analysis between four groups of ratsThere was no correlation between serum vaspin and FPG before surgery and after surgery in each group. serum vaspin levels in DRO group rats and in DSO group rats before surgery and four weeks, eight weeks after surgery correlated positively with those in INS and HOMA-IR(P < 0.05, respectively). serum vaspin levels in NRO group rats and in NSO group rats before surgery and four weeks, eight weeks after surgery had no correlation with those (P>0.05, respectively).Conclusion:1 RYGB surgery can reduce the fasting plasma glucose of type 2 diabetic rats, and treat type 2 diabetic rats, providing theoretical support for the wide clinical application of RYGB. 2 There is no marked changes of weight in type 2 diabetic rats before and after RYGB surgery ,which indicates the hypoglycemic effect is a direct result of surgical procedure, not secondary changes after weight loss.3 RYGB surgery has no obvious effect on blood glucose in normal rats, indicating that the effect of RYGB on the treatment of T2DM may be related to glucose tolerance.4 Elevated levels of serum vaspin and serum insulin levels correlated positively with those in INS and HOMA-IR in T2DM rats suggest that vaspin may be related to the pathogenesis of type 2 diabetes, which is expected to become a new target. Type 2 diabetic rats Serum vaspin levels and vaspin mRNA expression of adipose tissue were reduced and insulin resistance was improved after RYGB surgery, which may be one of the mechanisms of the treatment for T2DM.