| Abstact Objective To establish an autoimmune model of chronic abacterial prostatitis (CAP) of SD-rat and to investigate the difference and significant of intracellular calcium ion concentration ([Ca2+]i) of smooth muscle between the model group and the control group.Methods 50 rats were randomly divided into inflammatory group and the control group. 10 normal SD rats prostate protein were extracted and Purified, A model of CAP of SD-rat was established by purified prostate protein and Freund's complete adjuvant ( FCA) emulsion (1:1 suspension) injection, and normal SD rats injected with saline in the same way as a control group. 45 days after modeling, made a portion of each rat tissue HE staining, paraffin-embedded histological observation after the biopsy, after the model was successfully confirmed in vitro SD rat prostate smooth muscle cells, Both of the Prostate smooth muscle cells (PSMCs) were cultured in vitro and purified.Using immunohistochemical SP method proved out of the cells cultured smooth muscle cells; with the digestion cell suspension into the cell, the highly specific calcium fluorescent indicator Fluo 3-AM to join the cell suspension, the setting of carbon dioxide incubator and incubated for 1h load cells, centrifuged to remove excess dye, 3 times repeated washing by calcium-free buffer。The [Ca2+]i of PSMCs was assayed by the Fluo 3-AM calcium detection and flow cytometry. Results Comparison with both group, the date showed the value of the fluorescence intensity of the model group(65.11±14.78) was higher than that of the control group(45.36±4.19) with an emission wavelength of 525nm(P<0.05). The value of the fluorescence intensity was proportional with the [Ca2+]i.Conclusion The [Ca2+]i of PSMCs is higher in prostatitis group which may disturb the balance of the [Ca2+]I and affect the physiological function of the PSMCs, subsequently having a role of pathogenesis of the disease.
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