Relationship Between Monocyte Chemoattractant Protein-1 In The Gingival Crevicular Fluid And Periodontitis

Obiectives:Periodontal disease was the inflammatory diseases, which not only cause tooth loss, but also threaten human health, and monocyte chemoattractant protein-1(MCP-1) is one of specific indicator of inflammation. The purpose of this study is to analyse the feasibility of MCP-1 as an objective factor for periodontal destruction and inflammation degree.Methods:Forty nine case, including 20 males and 29 females, aged 21-60, were divided in four groups such as health group, gingivitis group, non-active periodontitis group, and active periodontitis group. The first or second mandibular molar was cloosen as subjects every case, the most serious postion of periodontal destruction, thus composing 49 samples. And clinical parameters, such as gingival index (GI), probing depth (PD), clinical attachment loss (CAL) were measured and analysed. Furthermore, GCF was collected and the concentration of MCP-1 was detected using ELISA method.Results:1 There was statistically significant difference among the active periodontitis group, the non- active periodontitis group, the gingivitis group and the periodontal health group.2 The volume of GCF in the active periodontitis group, the non- active periodontitis group, the gingivitis group and the periodontal health group were: 35.18±15.55μl , 21.09±10.90μl, 17.78±10.24μl and 12.00±6.89μl respectively(P<0.05).3 The average concentration of MCP-1 in the active periodontitis group, the non-active periodontitis group, the gingivitis group and the periodontal health group were 7.78±3.74×10^-3nmol/L, 10.89±3.65×10^-3nmol/L, 9.51±4.93×10^-3nmol/L and 14.20±5.48×10^-3nmol/L. Significant difference was found in the concentration of MCP-1 of the active periodontitis group when compare with others groups(P<0.05).4 It was correlated in the concentration of MCP-1 and the gingival index, the probing depth and the clinical attachment loss.Conclusions:1 It suggest that the content of GCF can be chose as one of reference indicator of periodontal condition.2 The level of MCP-1 in GCF of the active periodontitis is upregulated distinctly, and may be important factor for the pathogenesis and development of periodontitis. The concentration of MCP-1 in GCF can be regarded as a candidate indicator that reflect the status of periodontitis.3 There was statistical correlations between the concentration of MCP-1 in GCF and clinical parameters.