| Background and Objective: Thyroid carcinoma is the most common thyroid malignant tumor, accounts for about 95%, it is earliest reported in the early years of the 19th century. It didn't get the attention in a long time because of its low morbidity and mortality. It is described in HuangJiaSi surgery that thyroid carcinoma accounts for 0.3% (male) ~ 1.0% (female)of the whole malignant tumor, about 5% of the death case of cancer. The incidence of thyroid carcinoma is different with regional, age and gender. According to the statistics from the International Agency for Research on Cancer(IARC),in 90s of last century, the highest incidence is in Hawaii, the incidence rates of female (8/10million), male (2.9/10million). Yet the lowest incidence is in Poland, 1.4/10million for female, 0.4/10million for male. It is rare that the incidence of thyroid carcinoma in children who under the age of 15, girls are in about 0.22/10million, boys are about 0.09/10million. China is located in the low incidence area of thyroid carcinoma, lacking of comprehensive statistics. From 1983 to 1987, the results of the statistics of Tianjin showed that the annual average incidence of thyroid carcinoma is 1.49/10million, 0.9/10million for male and 2.0/10 million for female. It is reported in 1988 in Shanghai, the incidence of thyroid carcinoma, 0.8/10million for male, 2.2/10million for female. From the 1980s to the nowadays, the world statistics over about the past twenty years, thyroid carcinoma incidence increases, and mortality maintain stable. In 2006, The Journal of The American Medical Association reported that from 1973 to 2002, thyroid carcinoma incidence increased about 40%. British Association for Cancer Research(BACR) also released in 2006, the clinical data statistics in 2001-2005 show that the incidence of thyroid carcinoma increased from 1.4/10million in 1975 to 2.9/10million in 2006, male and female for 1:3 proportion incidence. China has recently reported in the literature to Hangzhou city, in 2009, thyroid carcinoma incidence rates of female malignant tumor row fifth. Shanghai thyroid carcinoma from 2002 total incidence 4.71/10million rise to 2006 9.69/10million male from 2002, the incidence of 2.27/10 million rise to 2006 4.06/10 million female from 2002, the incidence of 7.18/10million rise to 2006 15.37/10million. And because the part of thyroid carcinoma patients without clinical symptoms and signs or thyroid carcinoma with occult clinically found hard to thyroid carcinoma may be higher than the actual rate of clinical diagnosis. In short, with the understanding of disease, diagnostic technology improvement and enhancement of the doctor consciousness, patients with thyroid carcinoma incidence has shows ascendant trend. And so far, thyroid carcinoma etiology and pathogenesis is still not clear. District, age, gender, ionizing radiation, genetic, hormones and some thyroid diseases such as endemic goiter, adenomas are considered and thyroid carcinoma related factors. Further research with thyroid carcinoma, looking for the new factor is closely related, still very necessary.In recent years, with the increase of tumor incidence of research and development, people gradually means found virus infection and tumors have close relations, and become a new hotspot research tumor. Epstein first discovered EB virus (EBV) in Burkitt,s lymphoma in 1964 , after years of research, the first virus EB confirmed and herper virus. EBV is a widespread herpes virus, major violations of human B cells, B lymphocytes, epithelial and gland, etc have close, are now in force of nasopharyngeal carcinoma, Hodgkin's disease, and non-Hodgkin's lymphoma, stomach, lung, breast carcinoma organizations of the virus detected EBV successively gene product.1987 such reports its first begin with EBV of pulmonary lymphatic epithelial tumor about sample carcinoma; In 1994, HanGK reported EBV and Hodgkin's lymphoma relationship; In 1992 Darryl Shibata research reports of carcinoma of the stomach virus with EBC relevance. In 2006 Arbach Hartch reported EBV in breast carcinoma tissue virus gene expression. For EBV in China and abroad virus infection and the correlation of thyroid carcinoma happen very little, the reports of research in 2000 Zhang TingGuo who reported the thyroid carcinoma research in LMP-1 the expression of EBV and significance. This study aims to test whether thyroid carcinoma tissues, the virus has EBV thyroid carcinoma happened with EB virus infection is concerned whether.Methods: A total of collecting Hebei Medical University Second Hospital pathologists from October 2009 to February 2011 thyroid tumor resection specimen archive wax block 83 cases. For every case of samples after the conventional HE dyeing pair a pathologist physicians were observed pathologically and make diagnostic proof. According to the WHO thyroid tumor classification standards, divided into papillary carcinoma group follicular carcinoma, medullar carcinoma group, undifferentiated carcinomas group, at the same time in the thyroid gland tumor group and the pathological type thyroid carcinoma as compared to take corresponding group by group organized as tumor. Combined apply Immunohistochemical SP method to detect thyroid tumor organization and corresponding in normal tissue adjacent to tumor EBV -LMP1 and in-situ molecular hybrid technology testing thyroid tumor organization and corresponding tumors in normal tissue adjacent EBERs, in order to find EBV infection trace. Apply with SPSS 13.0, chi-square criterion, to analysis the results of statistical analysis.Results:1 The expression of LMP-1 in thyroid tumor LMP-1 positive signals at client cells cytoplasm, attached figure. The pathologic types see attached tabulation sheet to express positive. LMP-1 in the papillary carcinoma express positive rate 44.1%(15/34), follicular carcinoma in for were 36.4%(4/11), in anaplastic carcinoma for 16.7% (1/6), medullary carcinomas group 25.0%(1/4). The express positive rate of LMP-1 for male is 9.3%(5/17), the female is 42.1% (16/38).28cases of thyroid gland tumors seen LMP-1 4 cases were positive, positive rate 14.3%. Oncogene adjacent tissues all did not see positive expression. The pathological type thyroid carcinoma by Fisher between groups exactly chi-square criterion probability method there are no significant difference (P>0.05).LMP-1 indicating the expression and pathological types have nothing to do. Thyroid carcinoma and thyroid adenoma via the matching between chi-square criterion, indicates a significant difference (χ~2 = 5.034, P=0.025<0.05).LMP - 1 in the expression of thyroid carcinoma has nothing to do with sex organization (χ~2=1.216, P=0.371>0.05).2 The expression of EBER-1 in thyroid tumor tissue EBERs positive signal located in the host cell nuclei, show brown particle, attached figure, all the negative control and blank control results show no color, no background dyeing interference. The pathologic types see attached tabulation sheet to express positive. EBER-1 in the papillary carcinoma express positive rate 64.7%(22/34), in a follicular carcinoma for 45.5% (5/11), in anaplastic carcinoma for 50.0% (3/6), in medullary carcinomas group 25.0%(1/4). The express positive rate of LMP-1 in male is 52.9%(9/17), the female is 68.4%(26/38). 10 cases of EBER-1 positive in 28 cases of thyroid adenomas, 46.4%.Oncogene adjacent tissues all did not see positive expression. The pathological type thyroid carcinoma by Fisher between groups exactly chi-square criterion no significant differences between groups (P>0.05).EBER-1 has nothing to do with pathologic types of expression. Thyroid carcinoma and thyroid adenoma via the matching between chi-square criterion, indicates a significant difference (χ~2=5.828, P=0.016<0.05).EBER-1 in the expression of thyroid carcinoma has nothing to do with sex organization (χ~2=1.216, P=0.270>0.05).3 The positive of EBER-1 is 63.6% (35/55), and the positive of LMP-1 is 38.26% (21/55) in thyroid carcinoma tissues. The results of in situ molecule hybridization method and immunohistochemical method to detect thyroid carcinoma tissue is different by chi-square criterion , and in situ molecular hybrid method higher.Conclusion:1 Infection rate of EBV in thyroid benign and malignant tumor tissue is different, and in thyroid carcinoma tissues, EBV infection rate is higher, tip with the relationship between the thyroid carcinoma may be less than the thyroid gland tumors occur more closely.2 The pathological type of thyroid carcinoma is no significant differences in EBV infection, indicating that certain types carcinoma tissue is no concerned about EBV.3 According to the research in EBV infection , the detection rate of in situ molecules than immunohistochemical is higher, more sensitive.
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