Relationship Between EBV Infection And Different Types Of Lymphomas In Different Sites

Objective: As other malignant tumors, malignant lymphoma is caused by several factors among which the biotic factor plays an important role in the pathogenesis of malignant lymphoma. Epstein-Barr virus (EBV) is a kind of DNA virus related to the occurrence of many tumors. The aim of this experiment was to investigate the association between EBV infection and malignant lymphoma in Hunan province, based on the study of EBV infection status of malignant lymphoma in different sites and different types.Materials and Methods: Paraffin-imbedded Tissues in this experiment were mainly selected from three famous hospitals, including the First Affiliated hospital of Nanhua University (1993~2007, 55 samples), Hunan Cancer Hospital (2000~2007, 56 samples) and the First Affiliated Hospital of Xiangya(2001~2006,16 samples). These samples were composed of 60 nasal cavity and nasopharynx tissues, 30 stomach tissues and 37 superficial lymph node tissues.There were nineteen Hodgkin's lymphoma cases, in which including 15 males and 4 females. The male to female ratio was 3.75:1. The minimum age was 5 years and the maximum age was 65 years. The median age was 24 years. There were 108 cases of non-Hodgkin's lymphoma, in which including 66 males and 42 females. The male to female ratio was 1.57:1. The minimum age was 11 years. The maximum age was 76 years. The median age was 50 years.Applied S-P Immunohistochemistry, according to different markers, all 108 non-Hodgkin's lymphoma samples were divided into three immunophenotypes. B cell lymphoma with CD20 positive, CD45RO negative and CD56 negative was the first type. T cell lymphoma with CD45RO positive, CD20 negative and CD56 negative was the second type. NK/T cell lymphoma with CD45RO positive, CD20 negative and CD56 positive or negative was the third type.In situ hybridization was employed to detect the EBV-encoded small RNA (EBER) in malignant lymphoma, by which experimenters could determine whether EBV virus exist in malignant lymphoma. Moreover, the relationship of EBV infection in different types and different immunophenotype malignant lymphoma could be researched.Results:1. Nasal cavity and nasopharyngeal lymphomaThere were 35 EBER positive cases in nasal cavity and Nasopharyngeal and non-Hodgkin's lymphomas. The EBER-positive rate was 58.3% (35/60). The NK/T-cell lymphoma was 29 cases, including 19 EBER positive cases. The EBER-positive rate was 65.5% (19/29). The B-cell lymphoma was 31 cases, including 16 EBER positive cases. The EBER-positive rate was 51.6% (16/31).2. Gastric lymphomaThere were 30 Gastric non-Hodgkin's lymphoma cases, including 9 EBER positive cases. The EBER-positive rate was 30% (9/30). There were 28 cases of B-cell lymphomas, including 9 EBER positive cases. The EBER positive rate was 32.1% (9/28). There were 2 T-cell lymphoma cases and each was EBER negative.3. Lymphoma in superficial lymph nodeThere were 18 non-Hodgkin's lymphomas in superficial lymph nodes, including 2 EBER positive cases. There were 8 T-cell lymphoma cases, including one EBER positive case. There were 10 B-cell lymphoma cases, including one EBER positive case.There were 19 Hodgkin's lymphomas in superficial lymph nodes, including 5 EBER positive cases, accounting for 26.3% (5/19). There were 108 non-Hodgkin's lymphoma cases in all the samples which located at Nasal cavity and nasopharynx, stomach and superficial lymph nodes, including 46 EBER positive cases. The EBER positive rate was 42.6%.Conclusion:1.The present study shows that the EBV infection rate of non-Hodgkin's lymphoma was 42.6% and that of Hodgkin's lymphoma was 26.3% in Hunan , according to our study.2. The EBV infection rate in nasal cavity and nasopharyngeal non-Hodgkin's lymphoma is higher than that in the Gastric non-Hodgkin's lymphoma and in the superficial lymph node non-Hodgkin's lymphoma.3. There was no gender and age different significant of EB virus infection rate in lymphoma.