| Objective:1. To built a duck model of hepatits B combined with nonalcoholic fatty liver disease (NAFLD) by using a high-fat and high-sucrose diet.2. To research the influence on the blood lipid of ducks by antiviral therapy.3. To research if the steatosis will affect virological response in ducks.4. To find out whether lipid-lowering therapy can improve the virological response efficacy in ducks.Method:1.A hundred a day of age Guangdong sheldrakes were fed for three days. Sixteen of them which with congenital infection of duck hepatitis B virus (DHBV) were screened by polymerase chain reaction (PCR) method.3 weeks later they were divided into two groups equally, model group and control group. The control group was fed with normal fodder, the model group fed with a high-fat and high-sucrose diet which consist of 83% duck fodder,10% lard,5% sugar,2% cholesterol for 3 weeks, 100g/kg/d. The level of glucose (Glu), cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), DHBV DNA load in serum were determined at zero week, second week, third week, comparing the levels above and figuring out liver index, observing pathological steatosis changes of the liver tissue and making clear the changes of these indices.2. Fifty ducks with congenital infection of DHBV were screened by PCR method.3 weeks later, thirty-nine of them which were fed a high-fat and high-sucrose diet were divided into the groups of Entecavir and Kezhi capsule high/middle/low dose, Kezhi capsule high/middle/low dose; and eleven of them which were fed a normal diet were divided into virus control group and Entecavir control group. Each of them was treated with equal doses of Entecavir:0.1mg/kg/d,and (or) corresponding doses of Kezhi capsule: lOg/kg/d,5g/kg/d,2.5g/kg/d. The levels of glucose(Glu), alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), cholesterol (TC), glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), high density lipoprotein cholesterin (HDL-C), low density lipoprotein cholesterin (LDL-C), DHBV DNA in serum were determined at the time point of TO, T7, T14, T21 and P5. TC, TG, DHBV DNA in liver and the pathological changes of the liver tissue in inflammation and fatty deposition were also observed in all groups.Results:1.There were obviously statistic differences of biochemical results in serum between model group and control group after the model made (P<0.01).At the second week every index was higher comparing to the control group except TG (P<0.05,0.01). At the third week every index was significantly higher comparing to the control group (P<0.05, 0.01), transaminase kept higher comparing to the second week (P<0.05). There were no statistic differences of the liver index and DHBV DNA load in serum before and after the model made. Pathologicl results show that there were obvious sreatosis in liver tissue of the model group, moderate edema and inflammatory cells were found in the control group.2. There were no statistic differences of biochemical results in serum between Entecavir control group and virus control group; there were no significant differences of the ALT, AST level in serum compared Kezhi capsule groups with Enticavir and Kezhi capsule groups (P>0.05); the DHBV DNA level in serum was lower in the groups which were treated with antiviral therapy than those which were untreated (P<0.05); the decreasing DHBV DNA level in high dose Kezhi capsule and Entecavir group was more significant than the level in low dose group (P<0.05).3. Pathologically, the degrees of liver inflammation and steatosis were obviously better in Entecavir and Kezhi capsule groups than those in virus control group, and the inflammation was better than that in Kezhi groups, too; but the steatosis was evidently improving in Kezhi capsule groups compared with that in the combined treatment groups.Conclusion:1.The animal model of hepatits B combined with NAFLD could be built by using a high-fat and high-sucrose diet to feed three-week-old Gangdong Sheldrake for 3 weeks.2. There was no remarkable influence on the lipid of ducks in serum after the antiviral therapy.3. CHB combined with liver steatosis impacted on the virological response of Entecavir, and lipid-lowering therapy could improve the virological response efficacy.4. The antiviral and lipid-lowering treatment was better than a single therapy way on improving the liver inflammation.
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