Effects Of Acupuncture On IRS-1,IRS-2 And GLUT4 In Rats With Insulin Resistance

ObjectiveOur aim was to observe the effects of acupuncture on IRS-1, IRS-2 and GLUT4 of insulin resistance model rats' femoris muscle, so as to further explore its underlying mechanism.MethodsTwenty four male SD rats were divided randomly into three groups:the blank group, the model group and the acupuncture group. We induced insulin resistance model through the high glucose, high salt and high fat diet. From the sixth week, we tested FPG and FINS in the rat's blood every week, then figured out the ISI. When the ISI of the model group and the blank group was statistically significant (P< 0.01), the model was success. Then in the next three weeks, the rats of the blank group were fed by normal diet, while the rats of the model group and the acupuncture group were fed by the high glucose, high salt and high fat diet. The rats of the acupuncture group were treated by electroacupuncture at the same time, twenty minutes per day. We chose four points:Neiguan, Zusanli, Sanyinjiao and Shenshu.After three weeks of treatment, all rats were drew blood, preparing for testing FPG, FINS and C-P. Then they were perfused by insulin before being killed. Their quadriceps femoris muscle was cut off. Finally IRS-1, IRS-2 and GLUT4 were tested by methods such as RT-PCR, western blotting and so on.ResultsInsulin resistance rat model was built successfully. FPG and FINS of the model group rose evidently(P<0.01), ISI of the model group declined remarkably(P<0.01). After treatment, FPG, FINS, ISI and C-P of the acupuncture group improved evidently(P<0.01, P＜0.05). IRS-1 mRNA of the model group declined remarkably (P<0.01). IRS-1 mRNA of the acupuncture group rose evidently (P＜0.01).IRS-2 mRNA of the model group declined remarkably (P＜0.01). IRS-2 mRNA and IRS-2 protein expression of the acupuncture group rose evidently (P＜0.01). GLUT4 mRNA and GLUT4 protein expression of the model group declined remarkably (P<0.01). GLUT4 mRNA and GLUT4 protein expression of the acupuncture group rose evidently (P＜0.01).GonelusionIn this study, we found acupuncture's regulating action to PI3K route. We presume acupuncture treat insulin resistance model rats through increasing IRS-1 mRNA, IRS-2 mRNA and protein expression, GLUT4 mRNA and protein expression. The action mode and pathway of these protein were not clear yet, which need us to study further.