Effects Of Acupuncture On Signal Transduction In Rats With Insulin Resistance

ObjectiveOur aim was to observe the effects of acupuncture on PI3K-p85, PDK1, Akt2 and VKCλ/ζof insulin resistance model rats'femoris muscle, so as to further explore its underlying mechanism.MethodsTwenty four male SD rats were divided randomly into three groups:the blank group, the model group and the acupuncture group. We induced insulin resistance model through the high glucose, high salt and high fat diet. From the sixth week, we tested FPG and FINS in the rat's blood every week, then figured out the ISI. When the ISI of the model group and the blank group was statistically significant (P<0.01), the model was success. Then in the next three weeks, the rats of the blank group were fed by normal diet, while the rats of the model group and the acupuncture group were fed by the high glucose, high salt and high fat diet. The rats of the acupuncture group were treated by electroacupuncture at the same time, twenty minutes per day. We chose four points:Neiguan, Zusanli, Sanyinjiao and Shenshu. After three weeks of treatment, all rats were drew blood, preparing for testing FPG, FINS and C-P. Then they were perfused by insulin before being killed. Their quadriceps femoris muscle was cut off. Finally PI3K-p85, PDK1, Akt2 and PKCλ/ζwas tested by methods such as QT-PCR, western blotting and so on.ResultsInsulin resistance rat model was built successfully. FPG and FINS of the model group rose evidently(P<0.01), ISI of of the model group declined remarkably(P＜0.01). After treatment, FPG, FINS, ISI and C-P of the acupuncture group improved evidently(P<0.01,P<0.05).PI3K-p85a mRNA and PI3K-p85 protein of the model group over expressed(P<0.01), acupuncture made it descend to normal level.PDK1 protein expression of three groups was not statistically significant(P>0.05). Compare to the model group and the blank group, Akt2 mRNA of the acupuncture group rose significantly(P＜0.01).Phospho-PKCζ/λmRNA of the model group reduced remarkably(P<0.01). The acupuncture group's phospho-PKCζmRNA rose(P<0.01), but its phospho-PKCλmRNA had not changed(P>0.05). Phospho-PKCζ/λ. protein expression of three groups was not statistically significant(P>0.05).ConclusionIn this study, we found acupucture's regulating action to PI3K route. We presume acupuncture treat insulin resisitance model rats through reducing PI3K-p85a mRNA and PI3K-p85 protein expression, increase Akt2 mRNA and phospho-PKCζmRNA expression. The action mode and pathway of these protein were not clear yet, which need us to study further.