Experimental Study On The Effect Of Fluoritum In Anovulative Model Rats On INH, IGF-â…  Levels

Objective:This paper explores the material basis of the fluoritum's influence of the rat models with ovulation failure on ovulation inhibition(INH), insulin-like growth factor-I(IGF-I) and ovarian morphology. It provides the theoretical foundation for clinical treatment of ovulation failure.Methods:60 SD female rats of 24 days age were chose as research object, and were distributed into 6 groups at random, which were model control group, western medicine control group, fluoritum group, calcium fluoride group, norishing kidney prescription, and normal control group, each group had ten rats. Using the Bogovich method set a pathological rat model of ovulation failure, using the Clomiphene and kidney tonify and blood circulation simulating soup for contracting, and using the immunohistochemicalstaining observed the fluoritum's influence on the rat with ovarian failure part of the INH and IGF-I.Result:Compared with the normal group, the model group's intensity of expression of IGF-I and INH is higher. The difference is obvious (P <0.01). After the treatment, the intensity of expression of IGF-I and INH reduced obviously(P<0.01, P<0.05). It shows that the fluoritum,calcium fluoride, norishing kidney prescription and medicine can repress the intensity of expression of IGF-I and INH. the above four groups have no difference (P>0.05). This illustrates calcium fluoride and other microelement jointly take effect in reducing the intensity of expression of IGF-I and INH. The average of fluoritum rats ray density is obviously smaller than calcium fluoride rats, but it's not a significant difference.Conclusion:The above result of the experiment indicates that IGF-I and INH probably participate the nosogenesis of polycystic ovary syndrome (PCOS). Fluoritum can reduce the abnormal high expression in local ovary of ovulation obstruction rat, and attenuate the function of the IGF-I and INH in local ovary, and it is superior to CaF2 in the intensity. CaF2 and the other elements of fluoritum maybe restrain the level of IGF-I and INH. It shows that fluoritum probably has the function of endocrine hormone. It can improve the function of regulate of HPOA, and promote the growth of primary ovarian follicles to dominant follicle, but the foundation material of fluoritum needs further study.