The Research Of The Expression Of MiR-20a And The Relationship Between MiR-20a And Clinical Pathology In Gastric Cancer

Gastric cancer, which is the second cause of death in male patients with tumor and the third in female patients, is the forth high incidence in the global malignant tumor morbidity. The occurrence, development and transference of gastric cancer is a result of a variety of related gene disorders. However, the early diagnosis, the clinical treatment and the prognosis in gastric cancer remains to be further researched at present.MiRNA plays an important role in the transference of tumor in many studies, which provides new ideas for tumor metastasis related diagnosis and treatment. MiRNA participates in multiple links of tumor metastasis,which is a very complicated process. MiR-20a controls E2F1 through negative feedback, blocking the activity of inducing the apoptosis of cells and promoting the activity of cell proliferation which is mediated by c-myc, and further promotes the occurring and development of malignant tumor.Objective To discusses the malignant transformation process in the organization for early diagnosis and treatment in malignant tumor by researching the expression of miR-20a and analysising the relationship between miR-20a and clinical pathology in gastric cancer tissue. Methods Tissue samples were obtained from 47 patients under going gastrectomy for gastric cancer in Department of General Surgery, Jilin University during the period from December 2009 to October 2010. To screen differentially expressed miR-20a between gastric cancer tissue and adjacent non-tumor tissue.The expression of miR-20a was analyzed using Real-time PCR. Results 1. The expression of miR-20a was higer in 78.7%(37cases) of total samples and lower in 21.3%(10cases) of total samples in gastric cancer tissue than in adjacent non-tumor tissue. The relative expression of miR-20a was 3.5796±0.2819 in gastric cancer tissue and 2.4698±0.5423 in adjacent non-tumor tissue. The average ratio was 1.5148 times.2. There was no statistical difference of the expression of miR-20a among the different diameter of turner, the different pathologically classified group and the different Dukes' staging groups of gastric cancer by the statistical analysis(p<0.05). Conclusions 1. The expression of miR-20a in gastric cancer tissue was higher than in adjacent non-tumor tissue. Its probably mechanism was that miR-20a controls E2F1 through negative feedback, blocking the activity of inducing the apoptosis of cells and promoting the activity of cell proliferation which is mediated by c-myc, and further promotes the occurring and development of malignant tumor.2. There was no obvious correlations between the expression of miR-20a and the different pathologically classified group or the different Dukes' staging group of gastric cancer, which suggested that miRNA-20a played an important role in the starting of tumor, but was unclear about the relationship between miR-20a and clinical pathology in Gastric Cancer, which needed to further research.