| Miiller cells are the main retinal glial cells, involved in retinal neurons nutrition, metabolism and retinal signal transduction. Miiller cells synthesize and secrete various neurotrophic factors and cytokines, playing a role in protecting retinal neurons. It may be related with many retinal diseases caused by pathological damage of neurons, such as glaucoma, diabetic retinopathy, proliferative vitreoretinopathy and retinal detachment. Therefore, the control of Muller cell growth characteristics cultured in vitro provides the necessary preparations for retinal physiology and pathological study from the cellular level. Mouse and rabbit are the frequently-used animals for Muller cell study, the understand of the two different sources of Muller cells growth characteristics helps to understand Muller cell proliferation mechanism and lays foundation for further study of Muller cell's role at the development of retinopathy. So as to provide more clinical treatment feasibility.Objective:Establish Muller cells's the most suitable serum concentration, Understand the growth characteristics of Muller cells.Method:Establish primary culture system of rabbit and mouse retina Muller cell by using tissue piece suspension culture, and determinate the most suitable serum concentration of Muller cell's proliferation vigor in the containing of 0%,5%,10%,15%,20% and 25% serum concentration using the method of MTT. Draw the cell growth curve, calculate cell doubling time and mitotic index.Result:(1)Rabbit Muller cells proliferation vigor in the containing of 20% and 25% serum concentration was higher than the other group from the third day, there were statistically significant differences from the fifth day(P<0.05).While there was no statistically significant difference between the containing of 20%, 25% serum concentrations group(P>0.05). Mouse Muller cells proliferation vigor in the containing of 20% serum concentration was higher than the other group from the third day. (2) The result of counting and MTT were consistent, rabbit Muller cell growth curve was significantly increase from the second to the fifth day, there was no significant increased on the sixth and seventh day. there was no significant difference in growth curve between P1 and P2 generation, while P3 and P4 generation cell growth curve were significantly decreased; The result of counting and MTT were consistent, mouse Muller cell growth curve was significantly increased from the second to the fifth day, there was no significant increase on the sixth and seventh day. With the increase of passage the number of cell growth slowed down. There was no significant difference in growth curve from P1 to P3 generation, while cell growth curve was significantly decreased from P4 generation.(3) Rabbit Muller cell doubling time from P1 to P4 generation were respectly:1.8±0.15d,1.8±0.12d,3.0±0.69d,4.0±0.80d,Mouse Muller cell doubling time from P1 to P6 generation were respectly:2.1±0.17d,2.1±0.44d,2.1±0.13d,2.5±0.78d,3.0±0.81d,3.0±0.45d.(4) Rabbit Muller cell mitotic index rose in 1-4 days and declined in 5-7days, there was no significant difference in cell mitotic index between PI and P2 generation, while P3 and P4 generation cell mitotic index was significantly decreased; Mouse Muller cell mitotic index rose in 1-4 days and declined in 5-7days, there was no significant difference in cell mitotic index from P1 to P4 generation, while from P5 generation cell mitotic index was significantly decreased.Conclusion:1. The serum concentration of 20% is the most suitable serum concentration for the growth of retinal Muller cells.2. According to the growth curve P2 generation of retinal Muller cells, P2 and P3 generation of mouse Muller cells are the best choice for test.
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