Serum Metabonomics Study Of Uighur MODY Families Based On ~1H NMR And Analysis Of Nutrition Survey

Objective:To study the characteristics of serum metabolites changes in Uyghur families'with MODY; to understand the characteristics of Uyghur MODY family diet pattern through nutrition survey. Methods:We gathered two Uyghur MODY families composed of four generations from Kashgar region, Xinjiang Uyghur Autonomous Region, China. A total of 53 family members were gathered. Their general information, measured blood glucose levels, blood lipid levels, and blood pressure were analyzed. Using H Nuclear Magnetic Resonance (1H NMR) spectroscopy, serum metabolites were measured for each study participants. After having conducted data pretreatment on the spectrogram, orthogonal partial least squares discriminant analysis (OPLS-DA) was used to interpret data. We divided subjects into two groups according to blood glucose (normal and high), blood pressure, body mass index (BMI) levels, and compared the metabolites. We determined differences of serum metabolism components between each group's serum using pearson correlation coefficients with significant difference detection and two-dimensional spectrum technology; a cross-sectional survey on dietary pattern with a semi quantitative food frequency questionnaire was conducted among 4 Uyghur MODY families in Xinjiang. Results:①Isoleucine and tyrosine levels were decreased significantly (P<0.05) andα-glucose,β-glucose levels were increased obviously(P<0.05), when high blood glucose group compared with normal blood glucose group.②Citrate, phaseomannite,1-methyl histidine and tyrosine levels were all decreased significantly(P<0.05), When comparing serum metabolites between hypertension group and normal blood pressure group in Uyghur MODY family members.③No significant metabonomic changes were observed when comparing normal BMI group and high BMI group.④Cereals, livestock and poultry meat, milk, eggs were the main foods of Uyghur MODY family, fruits and vegetables, beans and products did not reach the recommended amount, almost did not eat fish and shrimp; All three major nutrients were excessive intaken. Calcium, vitamin C were lack of intake, P, Ka, Na, Mg, Fe intake exceeded. Conclusion:①The metabolites in the serum of Uyghur MODY family members were very different in different groups. Metabonomic changes of Uyghur MODY family with high blood glucose group indicated that patients with hyperglycemia may have long-term energy loss on a sustainable basis, followed by tricarboxylic acid cycle (TCA cycle) metabolism disorder and the affected metabolism, which reduces liver glycogen decomposition and muscle glycogen glycolysis. Differences in serum metabonomics of Uyghur MODY family hypertension group suggested that the TCA cycle metabolic disorder and the obstruction of fat metabolism in the patients of the Uyghur MODY families.②The diet pattern of Uyghur MODY family is unreasonable; these two Uyghur MODY families's diet pattern needs to be changed scientifically.