Significance And Expression Of AKT, P27^Kip1 And Cyclin E In Gastric Carcinoma

Objective: Gastric carcinoma is one of common malignant tumour in my country. Its occurrence is concerned with environment and heredity and is a path-process of multiple factor and stage. The occurrence and development of gastric carcinoma involves in anormal expression of many oncogenes and anti-oncogene. The genes expression is difference in different histology types and differentiated gastric carcinoma. It is much worth with study and research the expression of oncogenes and anti-oncogene in gastric carcinoma to evaluate its prognosis. This study aimed to investigate the expression of AKT protein, p27^Kip1 protein and Cyclin E protein in progress of occurrence and decelopment of gastric carcinoma and provided forcible experiment information for prevention, early diagnosis and treatment.Methods: The growth and cell cycle of MGC803 cells treated by LY294002 were observed by draw cellular growth curve and plate clone formation and flow cytometry. The phosphorylation of AKT protein and p27^Kip1 protein and the expression of Cyclin E protein were detected by immuocytochemistry and Western-blotting in MGC803 cells. The phosphorylation of AKT protein and p27^Kip1 protein and the expression of Cyclin E protein analyzed by immunohistochemistry in gastric tissue array included of normal gastric mucosa, carcinoma side tissue, atypical hyperplasia tissue, primary cancer tissue and lymphonode metastatic carcinoma tissue. Then we made use of statistics to analyze those dataes.Results: 1. After LY294002 treated MGC803 cells, the results of cellular growth curve showed that cell growth velocity were obviously depressed, compared with untreatment group. 2. The results of plate colony formation displayed that volume of cell clone shrinked and quantity of cell clone decreased after LY294002 treatmed MGC803 cells, compared with untreatment group.. 3. The results of flow cytometry (FCM) showed that G1 period rate of MGC803 cells gradually raised and S period obviously decreased after LY294002 treated MGC803 cells, compared with untreatment group. 4. The outcome of immunohistochemistry and Western-blotting discovered that the phosphorylation of AKT protein increased and the phosphorylation of p27^Kip1 protein decreased and the expression of CyclinE protein down-regulated in MGC803 cells treated by LY294002, compared with untreatment group. 5. The consequence of gastric tissue array stained by immunohistochemistry exhibited that the positive expression rate of phosphorylation AKT protein between primary cancer and lymphonode metastatic carcinoma and normal gastric mucosa, carcinoma side tissue and atypical hyperplasia was difference of statistical significance (P<0.01). The positive expression rate of phosphorylation AKT protein between normal gastric mucosa and atypical hyperplasia tissue was difference of statistical significance (P=0.26). The immunohistochemistry dyeing effect of postive expression rate of phosphorylation p27^Kip1 protein discovered difference of statistical significance among normal gastric mucosa, carcinoma side tissue, primary cancer and lymphonode metastatic carcinoma (P<0.01). The positive expression of CyclinE protein in normal gastric mucosa, carcinoma side tissue, primary cancer, lymphonode metastatic carcinoma and atypical hyperplasia tissue were detected by immunohistochemistry stain and positive rate was statistical significance among those gastric tissues (P<0.01). The positive expression rate of phosphorylation CyclinE protein between normal gastric mucosa and atypical hyperplasia tissue was difference of statistical significance (P=0.26).Conclusion:1. The cellular growth and proliferation activity of gastric carcinoma MGC803 cells treated by LY294002 were degraded and MGC803 cells were hold-up in cell cycle of G1/S period. 2. The phosphorylation of AKT protein was inhibited to result in overexpression of phosphorylation p27^Kip1 protein and low expression of CyclinE protein in MGC803 cells.3. The phosphorylation of AKT protein and p27^Kip1 protein and the expression of Cyclin E protein were related with occurrence, development and differentiation of gastric carcinoma. With the phosphorylation of AKT protein and expression of CyclinE protein increased, the phosphorylation of p27^Kip1 protein was decreased.