| Background Pain reflects a complex physiological and psychological activity, and is one of the most common clinical symptoms. Acupuncture is an effective way for treatment of pain. Ultrasound has the characteristics of acoustics and heat. According to the principle and method of acupuncture analgesia, using focused ultrasound to irradiate acupuncture point, it's analgesia effect has been discussed. This study was to discuss the effectiveness of the acupuncture point analgesia after irradiating by focused ultrasound, in accordance with the change rule of pain tolerance thresholds of bilateral ears of each rabbit. The"Zusanli"(ST36) was selected because of it's analgesia effect. Safety of focused ultrasound was proved by observing the histology characteristics of tissues of"Zusanli"(ST36).Part 1 A feasibility study of measuring pain tolerance thresholds of bilateral ears of each rabbit by Electronic Von Frey Objectives: To prove the feasibility of measuring pain tolerance thresholds of bilateral ears of each rabbit by Electronic Von Frey.Methods: Pain tolerance thresholds of a fixed point near the edge of ear vein of bilateral ears of each rabbit were measured by Electronic Von Frey. Each rabbit ear was measured 6 times from the 1st minute time point to the 6th minute time point.Results: Pain tolerance thresholds of 30 rabbits at 6 time points were not changed observably(P>0.05).Conclusions: Measuring pain tolerance thresholds of bilateral ears of each rabbit by Electronic Von Frey is feasible.Part2 Influences of pain tolerance thresholds of bilateral ears after irradiation in left Zusanli of rabbits by focused ultrasoundObjectives: To explore the effectiveness and safety of focused ultrasound for analgesia by observing the changes of pain tolerance thresholds of bilateral ears, mental state,and histology of each rabbit after a single irradiation by focused ultrasound in"Zusanli"(ST36).Methods: Thirty healthy Zelanian rabbits irrespective of gender were selected and randomized into 2 groups (n=15). Treatment of group A: the left"Zusanli"(ST36) of each rabbit was irradiated for 5 minutes by focused ultrasound with the power of 2 watts. Treatment of group B: the left"Zusanli"(ST36) of each rabbit was irradiated for 5 minutes without output of focused ultrasound. Pain tolerance threshold of a fixed point near the edge of ear vein of each rabbit was measured before treatment. Pain tolerance threshold of right ear of each rabbit was measured after 1 minute of treatment, and the left's of each rabbit was measured after 2 minutes of treatment. Tissues of skins and muscles of bilateral"Zusanli"(ST36) were taken, biopsy was performed with HE dyeing.Results: Pain tolerance thresholds of rabbits of group A were enhanced obviously after irradiating (P<0.01), while group B's were not changed observably (P>0.05). Pain tolerance thresholds of group A were significantly different with those of group B (P<0.01) after treatment. Rabbits of group A were in low spirits after irradiating, while group B's were not. Tissues of skins and muscles of bilateral"Zusanli"(ST36) were normal in both groups.Conclusions: Pain tolerance thresholds of two ears of rabbits are significantly enhanced after irradiating left"Zusanli"(ST36) within a short time by focused ultrasound. It prompts that focused ultrasound has an analgesic effect , and is safe.Part3 A Study of Changes of Pain Tolerance Thresholds of Bilateral Ears of Rabbits After Irradiation In Left Zusanli by Focused UltrasoundObjectives: To explore the feasibility of focused ultrasound for analgesia by observing the change of pain tolerance thresholds of bilateral ears of each rabbit after a single irradiation by focused ultrasound in "Zusanli"(ST36).Methods: Thirty healthy Zelanian rabbits irrespective of gender were selected and randomized into 2 groups (n=15). The left"Zusanli"(ST36) of each rabbit of group A was irradiated for 5 minutes by focused ultrasound with the power of 2 watts. The left"Zusanli"(ST36) of each rabbit of group B was irradiated for 5 minutes without output of focused ultrasound. Pain tolerance threshold of fixed point near the edge of ear vein of each rabbit was measured before irradiation. Taking 16 time points after irradiation from the 1st min point to the 16th min point,pain tolerance threshold of right ear of each rabbit was measured at the odd time points, and the left's of each rabbit was measured at the even time points. Tissues of skins and muscles of bilateral"Zusanli"(ST36) were taken, biopsy was performed with HE dyeing.Results: Pain tolerance thresholds of right ears of rabbits of group A were enhanced observably from the 1st min point to the 11th min point after irradiation (P<0.05), and in the 13th min point and the 15th min point they were not different from those before irradiation (P>0.05). The lefts'were enhanced observably from the 2nd min point to the 12th min point after irradiation (P<0.05), and in the 13th min point and the 15th min point they were not different from those before irradiation (P>0.05). Pain tolerance thresholds of right ears of rabbits of group A were enhanced observably than those of group B after irradiation in the 1st min point, the 3rd min point, the 5th min point and the 7th min point(P<0.05). The lefts'of rabbits of group A were enhanced observably than those of group B after irradiation in the 4th min point, the 6th min point, and the 8th min point (P<0.05). Pain tolerance thresholds of bilateral ears of rabbits of group B were not change obviously after irradiation(P>0.05). Rabbits of group A were in low spirits after irradiation, while group B's were not. Tissues of skins and muscles of bilateral"Zusanli"(ST36) were normal.Conclusions: Pain tolerance thresholds of two ears of rabbits are significantly enhanced at a certain time after irradiating left"Zusanli"(ST36) by focused ultrasound. It prompts that there is a really safety analgesic effect with the treatment of focused ultrasound.
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