Influence Of Maximal Intensity Training On TLR4 And Correlation Factors Of Rats In Liver

Objective:Through measuring indexes change of TLR4 signaling pathway in rat liver after different intensity training in order to obtain a further understanding of the intensity training induced rat liver damage molecular biological mechanisms.Methods:After 2 weeks adaptive training, rats were randomly assigned to three groups:Control group (C, N=10), intensity training group (I, N=15) and Maximal intensity training group (MI, N=15). Rats of I ran at the speed of 35m/min, increase the speed with 2m/min per 3min. When accelerating to a certain speed that some rats can't followed and slid from the treadmill for three times. Sound, light, electric stimulus and driving were of no effect. The speed before this one was determined as the maximum intensity. Rats ran at that speed for 3 min. Repeated this process for three times. Rest time than training time for 1:1.5. The training of group MI was the same as the groupⅠ, but suspended increasing speed when accelerating to a certain speed that some rats can't followed and slid from the treadmill for three times. Sound, light, electric stimulus and driving were of no effect. Rats ran at this speed until exhaustion. Rats were killed after 11 weeks. The level of TLR4 on the surface of rats monocyte, TLR4mRNA, P38 and JNK protein in rats liver and IL-6, TNF-αin rats serum were measured.Result:The level of TLR4 on the surface of rat monocyte:there was no clear difference between the three groups (p>0.05). The level of TLR4mRNA in rat liver:Compared to C group, that inⅠgroup (p<0.05) and MI group (p<0.01) were greatly decreased. The level of p-P38 protein in rat liver: Compared to C group, those in I group (p<0.01) and MI group (p<0.05) were significantly increased. The level of P38 protein in rat liver:there was no clear difference between the three groups (p>0.05). The level of p-JNK protein in rat liver:Compared to C group,Ⅰgroup (p<0.05) and MI group (p<0.01) were significantly increased. The level of T-JNK protein in rat liver:there was no clear difference between the three groups (p>0.05). The level of IL-6 in rat serum:Compared to C group, I group (p<0.01) and MI group (p<0.01) were significantly decreased. MI group was great higher thanⅠgroup (p<0.01). The level of TNF-a in rat. serum:there was no clear difference between the three groups(p >0.05).Conclusions:The level of TLR4 on the surface of rats monocyte, TLR4mRNA in rats liver and TL-6 in rats serum could be decreased by intensity training especially maximal intensity training, but those training had little influence on the level of TNF-a in rat serum. P38 and JNK in rats liver also could be activated by those training, and related to the grade of intensi ty. Maybe it is associated wi th the decline of liver antioxidant ability. However intensity to a certain extent will occur immunosuppression, then the level of TLR4 will increase.