The Significance And Expression Of GCDFP-15 And P63 In Paget Disease And Pagetoid Bowen Disease

Objectives: To evaluate the significance and expression of GCDFP-15 and p63 in Paget disease and pagetoid Bowen disease.Materials and methods: Choosing patients that have been diagnosed of Paget disease and pagetoid Bowen disease pathologically in the dermatology department of the second hospital of jilin university between 1996 and 2010. All patients were completed with clinical information. Patients with Mammary Paget disease(MPD) (ten in all) were all female; out of the fifteen patients with extramammary Paget disease(EMPD) fourteen were male. We use GCDFP-15-positive slide of Paget disease and p63-positive slide of Bowen disease as positive control respectively. Paget disease and pagetoid Bowen disease (PBD)treated with Phosphate buffered solution(PBS) as first antibody are made negative control. We carried hematoxylin-eosin(HE) staining and immunohistochemistry(SP) over these two type diseases with GCDFP-15 and p63,using DAB as the chromogenic agent. Results observation: GCDFP-15 is protoplasm-coloration and p63 is nucleus-coloration. Paget cells and pagetoid cells are the target cells and we judge the immunohistochemical result positive when they are stained brown.Results: 3 out of 10 MPD are GCDFP-15 positive, 4 out of 15 EMPD are GCDFP-15 positive. In all, 7 out 25 Paget disease are GCDFP-15 positive while all 11 PBD are GCDFP-15 negative. P63 expresses in none of the Paget cells of Paget disease while 10 out of 11 pagetoid cells in PBD.Conclusion: 1,The positive rate of GCDFP-15 is relatively lower in Paget disease than in pagetoid Bowen disease. When GCDFP-15 is positive, it can differentiate these two diseases. But when GCDFP-15 is negative, we can not tell. From our experiment, we can lead to a conclusion that GCDFP-15 has a low sensitivity in differentiating the two diseases.2,p63 is overexpressed in Bowen diseases and positive rate is much higher in pagetoid cell than in Paget cells. P63 is a preferred marker in differentiating the two diseases than GCDFP-15.