Expression And Clinical Significance Of FOXP3 T Lymphocyte Subsets In Nasal Polyps

Background and ObjectiveNasal polyps is a kind of chronic inflammatory disease of nasal cavity and nasal sinuses mucosa, the total number of its incidence is about 1%-4%. Nasal polyps usually occur in the lateral wall of nasal cavity and nasal roof, Second, ethmoid and membranous of maxillary sinuses are also seen, and the rate of its recurrence was high. The etiology and pathogenesis of nasal polyps are not yet clear, recent research indicates that it may be related to infection, allergy, immunology and cell factor and so on.Forkhead transcription factor p3 is a newly discovered transcription factor, its a multifunctional transcription factor which is related to the growth and development of CD4+ CD25+ Treg cells. FOXP3 has been shown specifically expressed in CD4+ CD25+ T regulatory cells, to some extent, it reflects the level and functional activity of the CD4+ CD25+ T regulatory cells.The purpose of this study is to analysis the expression and distribution of FOXP3,CD4,CD25+ Treg cells, to explore the effects in occurrence,development of nasal polyps, to determine its clinical significance.Methods50 cases of nasal polyps(the test group) and 20 cases of normal inferior turbinate mucosa (the normal group) underwent HE staining and immunohistochemical (SP method detected) in two groups to detect the expression of FOXP3, CD4 and CD8, using statistical packages of SPSS16.0,the datas will be studied by classification, analysis and contrast.Results1 The positive products of FOXP3 protein localized in the cytoplasm and (or)cytoplasm, and the color was clearly brownish yellow or brown particles. Under the microscope, the FOXP3 positive cell could be observed in both of nasal polyps and normal turbinate mucasa specimemts.2 The positive staining of CD4 protein and CD8 protein mainly located in the cell membrane, and were stained for brown yellow or brown particles. In nasal polyps, a large number of CD4 and CD8 positive lymphocytes can be seen, wich gathered in the skin and around the gland, and was scattered distribution in the interstitium. CD4+Tcells was significantly higher than the number of CD8+Tcells. In normal mucosa group, most of the slices can also be found, but the quantity of CD4+T cells and CD8+T cells is less which concentrated in the glands around the blood vessels, and scattered.3 Computer graphics analysis system detects FOXP3, CD4, CD8 average gray value(1) The difference of average gray value of FOXP3 between 50 cases of nasal polyps and 20 cases of inferior turbinate mucosa was statistically significant (p< 0.05). The difference of average gray value of FOXP3 between unilateral and bilateral nasal polyps was statistically significant(p<0.05). The difference of average gray value of FOXP3 between starting group and relapse group wa statistically significant (p<0.05).(2) The difference of average gray value of CD4 between 50 cases of nasal polyps and 20 cases of inferior turbinate mucosa was statistically significant (p< 0.05). The difference of average gray value of CD4 between unilateral and bilateral nasal polyps was statistically significant (p<0.05). The difference of average gray value of CD4 between starting group and relapse group wa statistically significant (p <0.05).(3) The difference of average gray value of CD8 between 50 cases of nasal polyps and 20 cases of inferior turbinate mucosa was statistically significant (p< 0.05). The difference of average gray value of CD8 between unilateral and bilateral nasal polyps was statistically significant (p<0.05). The difference of average gray value of CD8 between starting group and relapse group wa statistically significant (p <0.05). 4. By spearson correlation analysis, there exists the negative correlation between FOXP3 and CD4 in nasal polyps(r=-0.383,P=0.006); nor exists between CD8 and FOXP3 (r=-0.364,p=0.009).Conclusion1 The expression of FOXP3 in nasal polyps reduced, suggesting that the absence or dysfunction of FOXP3 plays an important role in the occurrence and development of nasal polyps.2 The FOXP3, CD4 and CD8 were involved in the formation of nasal polyps, and maybe related to the recurrence properties of nasal polyps.3 The expression of FOXP3 CD4 and CD8 in nasal polyps correlated to each other, indicating that the three proteins might collaboratively involved in the formation of nasal polyps.4 By detecting the expression of FOXP3 in nasal polyps, we can know the state of CD4+ CD25+ regulatory T cell infiltration in nasal microenvironment, and determine the state of local immune response in nasal polyps, which provide a new method of treatment for nasal polyps.