Recombinant Mice Of ¦£-interferon Prevention And Treatment Of Toxoplasma Gondii Infection Of The Fetal Immune Mechanism

Objective To explore the effects of rmu- IFN-y on T cell subsets in peripheral blood and decidual tissues of pregnant mice infected with toxoplasma gondii and on vertical transmission of toxoplasma gondii. Methods pregnant mice, infected with T.gondii, were injected intraperitoneally rmu- IFN-y and killed after 72 hours . The numbers of CD4+ and CD8+ T cells in peripheral blood and decidual tissues were detected by FACS, and the rate of abortion and amount of embryos were compared among groups. Pathological changes in uterus , placenta and embryos were observed through microscope. The incidence of maternal-fetus transmission was calculated by immunohistochemical staining of toxoplasma gondii special antigen. Results 1.The level of CD4+T cells , CD4+/ CD8+ both in peripheral blood and in decidual tissues of the group infected with T.gondii was lower than that of control group during the gestation (P<0.05 ) . The level of CD4+T cells, CD4+/ CD8+ in mice administered with different dosages of rmu- IFN-y rose up dramatically ( P<0.05 ) ,while the level of CD8+ T cells increased significantly only in the mice with higher dosage of rmu- IFN-γ (P<0.05 ) 2. The rate of maternal-fetus transmission of both two rmu- IFN-y groups was lower than that of infected group (P<0.05 ) .Through transmission electron microscopy ,we could find that there were fewer oxoplasma gondii in embryos of both rmu- IFN-y groups, and the majority of...