The Significance Of Leptin In Knee Joint Fluid And Serum

Backgroud Osteoarthritis (osteoarthritis, OA) is a kind ofdegeneration of articular cartilage, and bone damage characterized byhyperplasia of chronic joint disease. In the United States population overthe age of 50, the disease is the two place to led to the loss of labor,after cardiovascular disease, total has reached about 2100 people.Epidemiological investigations revealed that OA predilection in theelderly population in the USA.Over sixty ages, the knee osteoarthritisimaging abnormal incidence of 70%. In our country the OA is also occurredin elder .over 65 years old. the incidence rate above 50% also in peoplewho over 75 years the incidence rate above 80%. Therefore, OA isincreasingly becoming the disease that impact people's health and qualityof life of. However, early diagnosis of osteoarthritis has been a difficultproblem in medicine. In the present for the diagnosis of OA relies mainlyon imaging studies combined with clinical symptoms to a comprehensiveanalysis, but it is hard to diagnos early,thus we lost the opportunity ofearly treatment, leading to post-treatment ineffective. Studies have shownthat when OA occurs in the synovial fluid and articular cartilage willproduce some of the early metabolites (such as leptin, etc.), and can reachthe blood and urine through body fluids circulation. Therefore, we hopeto find these early metabolites to monitor the disease progresses.Objectives 1 To detect the knee joint fluid and serum leptin levelswith synovitis and articular cartilage damage relations between the twodiseases.2 The significance of knee joint fluid and serum leptin in the detectionand the early diagnosis of osteoarthritis.Methods Collected 93 arthroscopic surgery patients (95 knees) in ourhospital from April 2009 to May 2010 in which the clinical diagnosis ofOA patients (according to the 1996 American College of Rheumatologydiagnostic criteria on OA) 41 cases of 42 knees (OA group), the other 53 knees of 52 cases of knee joint diseases (non-OA group)), was detected byELISA in synovial fluid and serum leptin levels, and based on arthroscopicOuterbridge cartilage injury score, Ayral synovitis score Method toevaluate the degree of cartilage damage, the degree of synovitis With thet-test and pearson correlation analysis for statistical analysis.Results 95 cases of knee joint synovial fluid and serum leptinconcentrations (38.62±9.41ng/ml; 26.27±7.96ng/ml) than non-patientswith osteoarthritis concentration (16.70±6.19ng/ml; 19.38±7.60ng /ml) was significantly higher (p <0.05). 95 cases of knee joint fluid leptinconcentration was positively correlated with Ayral score (r = 0.678, p =0.000), and serum leptin concentration was positively correlated with Ayralscore (r = 0.517, p = 0.000); OA synovial fluid of patients leptinconcentration was positively correlated with Ayral score (r = 0.327, p =0.035); OA serum leptin concentration was positively correlated with Ayralscore (r = 0.333, p = 0.031);. 95 knee joint fluid leptin concentrationwas positively correlated with outerbridge score (r = 0.661, p = 0.000),and serum leptin concentration was positively correlated with outerbridgescore (r = 0.371, p = 0.000); OA synovial fluid of patients leptinconcentration was positively correlated with outerbridge score (r = 0.409,p = 0.007); OA serum leptin concentration was positively correlated withouterbridge score (r = 0.320, p = 0.039). Joint synovial fluid in OA patients(r = 0. 436, p = 0.004) and serum leptin concentrations (r = 0.348, p =0.024) leptin concentrations and body mass index (BMI) were positivelycorrelated.Conclusions 95 cases of knee arthroscopy in patients with knee fluidand serum leptin levels reflect the degree of synovitis disease .And italso can reflect the degree of cartilage damage.With the leptinconcentration increased both synovitis and cartilage damage were worse.Therefore, leptin can be used as a method to monitor the development ofOA and can be a effective means of evaluation.