The Expression Of P21ras , TBK1, Bcl-xl And Their Significance In Pancreatic Carcinoma.

ObjectiveTo study the expression of p21ras,TBK1 and bcl-xl, and study the relationship among the three ,with clinical stage, histological differentiation, lymph node metastasis, gender and age. provide a rich theoretical basis to find a new target for the treatment of pancreatic cancer. Methods1. Fifty cases of pancreatic cancer tissue specimens were sected serial for six-eight sheets. One part for HE staining and under light microscope for morphology observation, the other part for immunohistochemical staining. At the same time access to clinical data to determine the clinical stage.2. To detect fifty cases pancreatic cancer specimens and twenty cases of adjacent normal tissues of the protein expression of p21ras,TBK1 and bcl-xl by Two-step immunohistochemical method ,and comparative analysis the combined with clinical data.ResultsThe protein expression of p21ras, TBK1, and bcl-xl were 76% (38/50), 70% (35/50) and 66% (33/50) in 50 cases of pancreatic cancer. Adjacent normal pancreatic tissue, the three proteins positive rates were 0% (0/20), 20% (4/20) and 25% (5/20). The three Proteins were significantly in pancreatic cancer tissues higher than in adjacent normal pancreatic tissue, pancreatic cancer group and normal tissues was significant difference between groups, (P <0.05). TBK1, bcl-xl in different gender, age, had no significant difference between sites (P>0.05) .the degree of differentiation in different tissues was significantly different (P<0.05). And this study did not find the protein expression of p21ras with lymph node metastasis of pancreatic cancer was significantly associated (P>0.05). TBK1 and p21ras, TBK1 and bcl-xl expression was positively correlated (P <0.05); p21ras and bcl-xl expression was positively correlated (P <0.05).Conclusion .1. The overexpression of TBK1, bcl-xl in pancreatic cancer, and with differentiation, clinical stage and lymph node metastasis, suggesting that TBK1 involved in pancreatic cancer development, invasion and metastasis process, may be important for tumor markers and indicators of malignant pancreatic cancer, suggesting that inhibition of bcl-xl and pancreatic cancer cell apoptosis.2. The overexpression of p21ras and overexpression of TBK1,bcl-xl are positive correlation, suggesting that K-ras gene mutation may be increased TBK1 and bcl-xl over-expression, TBK1 may induce K-ras gene mutations in tumor development has played a key effect.