Expression Of HTERT, C-myc And Ki-67 In Human Ovarian Granulosa Tumor And Their Clinical Significance

Background and Objective: Granulosa cell tumor of the ovary is rare neoplasms thatoriginate from sex-cord stromal cells. They represent about 2-5% of malignant tumors of theovary. The molecular pathogenesis of this kind of tumor is unknown. hTERT is humantelomerase reverse transcriptase, its expression has related to the activity of telomerase. c-mycprotein,a transcription factor encoded by myc protooneogene,also play a role in the positiveregulation of hTERT expression. Ki-67 is a cell proliferation nuclear antigen, which isrecognized as a marker which reflecting cell proliferation level. The purpose of the study is todefine the role of hTERT,c-myc and Ki-67 during the evolution of ovarian granulosa cell tumor.Methods The paraffin section of 35 diagnosed as ovarian granulosa cell tumor werecollected. Expression of hTERT,c-myc and Ki-67 was detected by immunohisto chemistry ,stainand compared with normal ovarian tissue.Results The expression of Ki-67,and hTERT in OGCT were higher than in normaltissues. The expression of Ki-67 and hTERT gradually increased in clinical stageⅠ,Ⅱ,Ⅲ.Theexpression of hTERT gradually increased when the differentiation of tissue decreased. Theexpression of Ki-67 in the of karyokinesis<3/10HPF was lower than in karyokinesis≥3/10HPF .There is a relationship between Ki-67 and hTERT in OGCT.Conclusions hTERT and Ki-67 can be take as indicator of the OGCT, and theirexpression can provide a theoretical basis for predicting biological behavior of OGCT.