The Expression And Regulation Of Th17 Cell In Murine Modal Of Allergic Rhinitis

Objective As the novel identification of helper T(Th)cell subsets, Th17 cells has been witnessed new insight for the molecular mechanisms in the development of immune response infections and autoimmune diseases through producing IL-17A,IL-17F,IL-22 and other cytokines, and thus led to revision of the classic Th1/Th2 paradigm. Currently, few researches had studied about the Th17 cells in allergic rhinitis. The mechanisms of allergic disorders were not clear. To study the symptoms, pathologies and the expressions of immunology effective cytokines of Th1, Th2 and Th17 cells, and the specific transcription factor of Th17 cells in normal mice, murine model of allergic rhinitis and application of IL-17 antibody in allergic rhinitis mice, thus to evaluate the role of Th17 cells in allergic rhinitis pathogenesis, and the potential treatment for the patients.Methods Thirty BALB/c mice half male and half female were randomly divided into control group, allergic rhinitis group, and IL-17 anti-body therapy group. There were ten mice in every group. The allergic rhinitis model was induced in allergic rhinitis group, and IL-17 anti-body therapy group by classical method with ovalbumin (OVA). Then, the therapy group was treated with IL-17 antibody. The control group was deal with physiological saline instead by the same method. The concentrations of Interferon-γ(IFN-γ), IL-4 and IL-17 in serum was measured by enzyme-linked immunosorbent assay (ELISA). The expressions of Retinoic acid-related orphan receptorγt (RORγt) mRNA were measured by reverse transcription-polymerase chain reaction (RT-PCR)on the nasal mucosal. Meanwhile, Nasal mucosal inflammation was evaluated by hematoxylin-eosin (HE) staining.Results Being induced, the mice in model group and intervention group appeared typical symptoms of allergic rhinitis. The model of allergic rhinitis is successfully established according to the grading standard. After the IL-17 anti-body intervention, the mice has fewer sneeze, occasional runny nose, and little scratch. Symptom grading is higher in the model group than the control group and the intervention group(q=10.22,9.10, P<0.05). The level of IL-17, IL-4 and RORγt mRNA of allergic rhinitis group were significantly higher than those of control group (q/Z=14.6,16.58,8.19, P<0.05). IFN-γwas on the contrary (q=8.02, P<0.05). The level of IL-17,IL-4 and RORyt mRNA were lower in the IL17-antibody treated group than the model group (q=11.03,13.9,6.95, P<0.05), and the level of IFN-γwas higher (q=6.94, P<0.05). Pathological inflammation response has been seen in the model group, like disruption in cilia, Goblet cells increased, expansion of Vasodilation and glandular hyperplasia etc. The inflammation in therapy group was reduced by nasal mucosal HE staining.Conclusion In addition to the Th1/Th2 model, the expression and function of Th2, Th17 cells in allergic rhinitis increases. It might be associated with the pathogenesis of allergic rhinitis. The control of Th17 cells may be an effective way to treat allergic rhinitis.