The Study Of Effect Of Gypenoside On The Expression Of T-lymphocyte Substock And Antioxidant Enzymes In Rats With Recurrent Oral Ulceration

Objective:Established the rat's recurrent oral ulceration(ROU)models and discussed the relationsip between T- lymphocyte substock,oxygen radical and the pathogenesis of recurrent oral ulceration and evaluated the immune regulation and antioxidative effects of Gypenoside(GPs) on ROU.In order to provide certain theory basis of Gypenoside(GPs)'s experiment and clinical research.Methods:Rats models of recurrent oral ulceration with immunology were established(Add Freund's complete adjuvant(FCA) to galacta of health variant rat's oral mucosa as the antigen, and injected it to two sides of spinal column);Cut the oral mucosa of normal and ulcer rats,and make Hematoxylin-eosin(HE) staining pathology examination;The rats were randomly divided into 4 groups,including control group,modelgroup,Levamisole group and Gypenoside group. Drawing blood from the eyes of rats while around the model was established and after administration.The changes of CD4~+,CD8~+ in blood were observed using flow - cytometre before and after the model as well as after the therapy.The activity of superoxide dismutase(SOD) was measured in all specimens of rats by xanthine oxidase method.The activity of glutathione peroxidase(GSHPx)was measured in all specimens of rats by DTNBdevelopment process.The activity of catalase(CAT) was measured in all specimens of rats by visible spectrometry.The contents of malondialdehyde(MDA) was measured in all specimens of rats by thiobarbituric acid method.Results:1.Histologically,infiltration of intraepithelial lymphocytes,dissolved ulceration,a large number of inflammatory cell infiltration in lamina propria,capillary congestion expansion,gland hyperplasia,the ulcers were formed.2.Compared with control group, the values of CD4~+and CD4~+/CD8~+ showed significantly lower level in ROU rats (P<0.05),while CD8~+ not.After Levamisole and Gypenoside treatment,the values of CD4~+ and CD4~+/CD8~+ increased significantly (P<0.05),It did not show any significant difference among the two groups.3.The activity of SOD and GSHPx of ROU rats was significantly lower than the control group (P<0.05).The contents of MDA was significantly higher than the control group(P<0.05).After treatment,The activity of SOD and GSHPx of Gypenoside group was significantly higher than the control group(P<0.05).The contents of MDA was significantly lower than the control group (P<0.05).The activity of SOD of Levamisole group was higher than the control group,but no significant difference.The activity of GSHPx and the contents of MDA was lower than the control group,but no significant difference.Conclusion:1.Compared with control group,the values of CD4~+ and CD4~+/CD8~+ showed significantly lower level in ROU rats,suggesting that The unbalance of T-lymphocyte substock may contribute to ROU incidence.2.The activity of SOD and GSHPx of ROU rats was significantly lower than the control group. The contents of MDA was significantly higher than the control group,suggesting that oxygen radical may contribute to ROU incidence.3.GPs had the immune regulation and antioxidative effects on experimental ROU rats,and the immune efficacy is as same as Levamisole,but the antioxidative effects excelled Levamisole.4.GPs had definite developing prospect for curing ROU.