Study On The Relationships Of The Oral Manifestations And Blood And Salivary HNP1-3,HBD-2 Expression In HIV/AIDS Patients

Objective: (1) To observe the expressions of HNP1-3, hBD-2 in the serum and saliva of HIV/AIDS patients, and to investigate their relationships with oral manifestation in HIV/AIDS patients. (2) To explore the relationship among HNP1-3 and hBD-2 expressions and blood CD4 count and oral health in HIV/AIDS patients,and to understand the role of HNP1-3 and hBD-2 in the oral innate immunity.Materials and methods: (1) The peripheral blood and saliva samples of 34 patients with HIV infection (HIV group), 36 patients with AIDS patients receiving ART treatment (AIDS treatment group), and 31 healthy donors (control group) were collected respectively and the oral health, condition and the clinical indicators were record. (2) To investigated the CD4 expression by using flow cytometry. (3) The peripheral blood and saliva samples of HIV/AIDS patients and healthy donors were investigated for the expressions of HNP1-3 and hBD-2 using enzyme-linked immuno-sorbent assay. Results: (1) Oral health: oral expression of HIV group including oral candidiasis (6 cases), xerostomia (7 cases), oral ulcer(6 cases), periodontal disease(2 cases), herpes simplex(1 case) and other disease (5 cases); 16 patients of AIDS group complained of dry mouth, oral expression including oral candidiasis (4 cases), xerostomia (12 cases), oral ulcer(2 cases), periodontal disease(7 cases), and other disease (3 cases); there were statistically significant (P <0.05) compared with the control group. (2) The level of defensins in saliva and blood: HNP1-3 and hBD-2 were expressed both in peripheral blood and saliva of HIV/AIDS patients and healthy controls. The median concentrations in HNP1-3 in the peripheral blood for HIV group sites (363.41ng/ml) and AIDS treatment group sites (257.17ng/ml) were greater than that of healthy sites (94.43ng/ml, P<0.05). The median concentrations of HBD-2 in the peripheral for HIV group sites (4.05ng/ml) and AIDS treatment group sites (3.75ng/ml) were greater than that of healthy sites (2.40ng/ml, P<0.05). The median concentrations of HNP1-3 in the saliva for HIV group sites (50.96ng/ml) and AIDS treatment group sites (33.63ng/ml) were greater than that of healthy sites (16.63ng/ml, P<0.05). The median concentrations of hBD-2 in the saliva for HIV group sites (33.22ng/ml) and AIDS treatment group sites (28.24ng/ml) were greater than that of healthy sites (20.13ng/ml, P<0.05). Compared to blood HNP1-3 and hBD-2 levels, the saliva HNP1-3 and hBD-2 levels that in HIV group, AIDS treatment group and control group were not statistically significant (P> 0.05). (3) The relationship among the defensins evel,CD4 count and oral representation: there is a negative correlation between the CD4 count and the expressions of HNP1-3 in blood and saliva (P<0.05), oral characterization and CD4 count with a negative correlation relationship (P < 0.05). there are a positive correlation between oral representation and HNP1-3 in saliva and blood and hBD - 2 in blood (P < 0.05) by Spearman 's method. (4) The relationship between the defensins level and oral representation: there were no significant difference between patients with oral characterization and without oral characterization (P> 0.05).Conclusions: (1) The oral Characterization of HIV / AIDS patients are oral candidiosis,xerostomia , HIV- parodontopathy,HIV-P ect. (2) The small amount of expressions of HNP1-3 and hBD-2 in healthy people suggest that they may play a role in maintaining the health of the body and oral mucosa as a part of innate immunity.blood and saliva in healthy patients have a small amount of HNP1-3 and hBD-2 expression, suggesting that HNP1-3 and hBD-2 innate immunity as part of the body in maintaining normal immune has a role. (3) The expressions of both HNP1-3 and hBD-2 in HIV / AIDS patients were higher than health controls may suggested that HIV/AIDS patients have a increase ability to produce HNP1-3 and hBD-2 expression in the blood and saliva. High level of HNP1-3 and hBD-2 expression in the blood and saliva sites may represent a biological factor that contributes to HIV/AIDS. (4) There is a negative correlation between the expressions of HNP1-3 and hBD-2 and CD4 count may hint us that the cellular immune function in patients with relatively high in the case of the levels of the induced defensins were low.