Experimental Study On The Chemotherapeutic Sensitivity Of Human Astrocytoma Cells In Vitro

Objective: To detection the sensitivity of usual chemotherapeutic drugs(BCNU, VM26, ADM, CDDP, CTX) used alone or combination to astrocytoma (AT) cells with MTT assay and compare the drug sensitivity to different types of AT cells(HGA, LGA, primary, recurrent),expecting to provide a reasonable basis for personalized post-operative chemotherapy.Methods: 44 different types of AT samples (pathologically confirmed AT) were cultured in CO2 training system , isolated and purified with differential—adhesion method and identified by immunofluorescence assay in detecting of glial fibrillary acidic protein (GFAP), then added different concentrations chemotherapy drugs, tested optical density (OD value) with MTT colorimetric assay and calculated their sensitivity rates.Results: 1. 38 AT samples were successfully cultured,and the success rate was 86%, which was the highest in HGA cells; 2.The sensitivity rates of chemotherapy drugs of AT samples are different and the inhibition range are great varying; 3.The combination of two therapy agents,which with different mechanisms or different cell-cycle targets,showed more sensitivity than single one (p <0.01); 4.The sensitivity rates of drugs in the high-grade and recurrence group were higher than that in the low -grade and primary group( p <0.05), but there were no statistical significance in combination chemotherapy to high-grade and low-grade group or primary and recurrent group (p> 0.05).Conclusions: 1. The success rate of AT cells culture is closely related to pathological types of tumor; 2.The sensitivity of chemotherapeutic drugs to AT cells vary widely , even the same type of tumor (pathological grade is like)has different sensitivity rate to the same drug; 3.Combined chemotherapy can increase the drug sensitivity rate; 4.Antineoplastic drugs have high sensitivity to high-grade and recurrent AT; 5. The sensitivity antineoplastic agents of clinical personalized chemotherapy may be selected according to the results of drugs test so as to increase treatment effect, decrease side effects and tumor recurrence.