In Vitro Study On The Effect Of Grape Seed Proanthocyanidin Extract (GSPE) On Actinomyces Viscosus

Objective:In vitro experimental studies on grape seed proanthocyanidin extract (GSPE) affecting Actinomyces viscosus growth and its acid metabolism and adhesion. Explore the impact of grape seed proanthocyanidins on the possible mechanism of caries-preventive agents for the new research and development, application and provide experimental evidence.Methods:1. Selecting Actinomyces viscosus ATCC15987 as the experimental strain. Investigating the impact of GSPE on bacterial growth, the liquid dilution method and plate method grape seed proanthocyanidin extract the minimum inhibitory concentration (MIC); selected Actinomyces viscosus 1/2MIC of GSPE in the cultivation of centrifugal after the biomass, after fixation, embedding a series of treatment, GSPE were observed on transmission electron microscopy of such morphological changes of bacteria.2. Using isotope labeling was observed Actinomyces viscosus on the different concentrations of GSPE treated saliva-coated hydroxyapatite (SHA) Inhibition of adhesion;3. GSPE the MIC value by measuring the following six concentration gradient effect on Actinomyces viscosus to calculate bacterial culture supernatant fluid changes in the value ofΔpH observed on Actinomyces viscosus acid metabolic effects.Results:1. When the concentration of grape seed proanthocyanidin extract is equal to or less than 16.00mg/ml when, GSPE on the growth of Actinomyces viscosus has a certain extent. Observed under transmission electron microscope 1/2MIC of GSPE on Actinomyces viscosus morphological changes, TEM view of Actinomyces viscosus cell swelling, cell wall damage.2. GSPE less than the following 1/2MIC Actinomyces viscosus can inhibit the adhesion of the SHA.3. GSPE concentrations equal to or less than 8mg/ml the role of the concentration gradient of Actinomyces viscosus, the culture medium supernatantΔpH value decreased, indicating that GSPE can inhibit the metabolism of Actinomyces viscosus acid;Conclusion:The experimental results showed that when the concentration of grape seed proanthocyanidin extract 16.00mg/ml sticky when that is able to inhibit the growth of actinomycetes, and to take the role of the minimum inhibitory concentration below the concentration of Actinomyces viscosus, when in its virulence factors of cariogenic adhesion, acid production were significantly inhibited, it prompted grape seed proanthocyanidin is expected to play a role in the prevention of dental caries.