| Objective:To establish a simple practical animal model of vertebral artery insufficiency, and study the causes and pathogenesis of cervical vertigo.Methods:In adult healthy Japanese white rabbits for the experimental study, through ligating and cutting of posterior cervical vertebrae muscles of C4-5, and removal of supraspinatus and the interspinous ligament of C4-5, then destroying anterior longitudinal ligament and intervertebral disc structure of C4-5 to establish animal model of vertebral artery insufficiency. The rabbits were randomly divided into two groups (n = 30): experimental group, were to ligate and cut posterior cervical vertebrae muscles of C4-5,and remove supraspinatus and the interspinous ligament of C4-5, and then destroy anterior longitudinal ligament and intervertebral disc structure of C4 - 5. Control group, were to cut and sew the neck skin,the incision length as the same as experimental group's,and without other surgery. Experimental group and control group rabbits, respectively, were to take over-straight and over- inflec- tional cervical X-rays when on the first 30 days,the fist 60 days and the first 90 days, and the relative range of cervical spine motion of 4 was measured. Color Doppler ultrasound were applied to test arteria vertebralis pulsatility index(Pi), resistance index(Ri) and blood flow. All the experimental group and control group rabbits were killed when the experimental group on the first 90 days. Specimens of vertebral artery were got to produce pathological section and then were stained by hematoxylin eosin(HE). After that vertebral artery pathological section were to observe. Computer image analysis system were applied to measure vertebral artery external diamete(ED)r, inner diameter (ID) and wall thicknesses(WT). SPSS10.0 software was used to carry out statistical analysis.The experimental datas were expressed with x±s.Results:The relative ranges of 4th cervical spine motion in Experimental group rabbits and control group rabbits were not statistically significant after the first 30 days, the first 60 days (P > 0.05); The relative ranges of 4th cervical spine motion in experimental group rabbits were significantly higher than the control group after the first 90 days ,the differences were significant (P <0.01). The mean of Pi in the experimental group was 0.43±0.03, 0.45±0.05 after the first 30 days, the first 60 days, control group, the mean Pi after the first 30 days, the first 60 days was 0.42±0.04, 0.44±0.06, the two groups showed not statistically significant (P > 0.05), the experimental group Pi mean the first 90 days after operation was 0.49±0.07, control group, the mean Pi in 90 the first days was 0.43±0.05, the two groups were statistically significant (P <0.05). Ri mean in the experimental group after the first 30 days, the first 60 days was 0.36±0.04,0.39±0.04, mean of the control group Ri in 30 days, the first 60 days was 0.37±0.06,0.38±0.05, the two groups showed no statistically significant (P > 0.05); experimental group Ri mean the first 90 days after operation was 0.43±0.07, mean of the control group Ri in 90 days was 0.38±0.03, the two groups were statistically significant (P <0.01) . Blood flow in the experimental group, mean after the first 30 days, the first 60 days was 4.21±1.32 ml / min, 3.96±1.45 ml / min, control group mean Blood flow in the first 30 days, the first 60 days was 4.19±1.48 ml / min, 4.17±1.31 ml / min, the two groups were not statistically significant (P > 0.05); Mean Blood flow in the experimental group after the first 90 days was 3.47±1.02 ml / min, the control group mean Blood flow in the first 90 days was 4.08±1.44 ml / min, the two groups were statistically significant (P <0.01). Control group, levels of vertebral artery biopsy showed clear, single-layer endothelial cells lining could be seen covered; experimental group, slices of vertebral artery showed a narrow lumen, intimal hyperplasia, medial muscle cell proliferation significantly, the wall was significantly thicker. Average vertebral artery diameter in the experimental group was 603.94±27.48μm after the first 90-day, in the control group was 608.73±31.37μm after the first 90-day, the two groups were not statistically significant (P > 0.05); experimental group of vertebral artery diameter after the first 90 days, an average of 407.87±28.42μm, control group, vertebral artery diameter in the first 90-day average of 492.39±34.17μm, a significant difference between the two groups, with statistical significance (P <0.01); experimental group of vertebral artery wall thickness after the first 90-day average of 98.47±17.28μm, control group, vertebral artery wall thickness in the first 90-day average of 58.72±13.81μm, a significant difference between the two groups, with statistical significance (P <0.01).Conclusions:1.Through cutting and ligation of posterior cervical vertebrae muscles of C4-5,and removal of supraspinatus and the interspinous ligament of C4-5, then destroying anterior longitudinal ligament and intervertebral disc structure of C4-5 to establish animal model of vertebral artery insufficiency,which was successful. It was similar to the clinical course of patients with cervical vertigo.The operation was simple and had high successful rate.2.The cervical spine abnormal motion played an important role in the vertebral artery insufficiency.3. The structure of vertebral artery was abnormal when vertebral artery insufficiency...
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