The Research Of The Fibrous Ring's Histology And Immunology Changes In Their Spinal Canal Of New Zealand White Rabbits

Objective:To observe the possible mechanism of resorption in the Free fibrous ring by making models of Lumbar Disc Herniation (LDH), so as to provide a new method and basics for conservative treatment of some certain types of LDH tissues.Design Setting and Time:Randomized controlled animal, experiments were performed at the department of anatomy of Yan Bian University and the department of pathology of Yan Bian University Hospital from October 2009 to March 2010.Material:Ninety adult and healthy New Zealand rabbits (irrespective of gender) were randomly devided into the model group (n=60),sham operation group (n=24) and control group (n=6). Methods:Sixty rabbits were randomly selected to establish the spontaneous LDH models. The animals in sham operation group were only exposed intervertebral discs. Rabbits in the model and sham operation groups were divided into 6 groups, and were sacrificed at 2,4,6,8,10,12 weeks after operation respectively. The specimens were observed and analyzed immunohistochemically for the presence of CD68+cells. The normal free fibrous rings were harvested from the animals of the control group and the same immunohistochemical detection was performed.Results:The various contraction and disappearance of the free fibrous rings were observed in the model group at different weeks after operation. The infiltration of CD68+cells was presented in the free fibrous rings of all the specimens, and the degree of infiltration was decreased as the time prolonged. But there were no CD68+cells in the free fibrous rings of the sham operation and control groups.Conclusion:...