Study On Chronic Prostatitis And Urinary Obstruction In Sprague-Dawley Rats Models

Objective:To investigate the influence of the ratio between testosterone and estradiol in serum on rats with chronic prostatitis and urinary tract obstruction. To detect the expression of COX-2 in model group and control group prostate tissues by immunohistochemical method.Methods:Adult Sprague-Dawley rats (n=30) were randomly divided into control group and model group. After being performed sham surgery,the rats of control group were given subcutaneous injection of peanut oil 1.0ml/kg everyday.The rats of model group were given subcutaneous injection of Estradiol Benzoate 0.25mg/kg (1.0ml/kg) everyday. A month later, two groups of rats were both carried out urodynamic examination and the rate between testosterone and estradiol in serum. The rats were killed to obtain the prostate tissue. HE stainings of prostate tissue was observed in model group and control group in order to study the pathological changes. To detect the expression of COX-2 in model group and control group prostate tissues by immunohistochemical method.Results:It is showed by histopathological examinations that the inflammation in prostate was significantly enhanced and the ratio was significantly reduced in model group comparing with control group. However,there is no obvious signs of urinary tract obstruction. The expression of COX-2 was weak in both prostate tissues of the model group and control group, no significant difference, P>0.05.Conclusion:The ratio between testosterone and estradiol in serum was significantly decreased in rats with estrogen-induced prostatitis. After Estradiol Benzoate subcutaneous injection for 4 weeks, no obvious signs of urinary tract obstruction were observed. The expression of COX-2 was weak in the estrogen-induced chronic prostatis in rat.