| Objective:To investigate the expressions of CD44 and EpCAM and its clinicopathologic significance in gastric adenocarcinoma. Furtherly to examine the locations, quantity of EpCAM and CD44 double positive cells in nomal gastric mucosa, adenoma and adenocarcinoma and to analyze their relationship with clinicopathologic factors. To examine the expression of bone marrow-derived mesenchymal stem cells maker CD166 and CD271 in nomal gastric mucosa, adenoma and adenocarcinoma and to investigate the relationship between the expression and the carcinogenesis and the value of CD 166 and CD271 as gastric adenocarcinoma stem cell maker. To examine the expression of CD44, EpCAM, CD166 and CD271 in human gastric adenocarcinoma cell line BGC-823 and to observe morphologic characteristics of positive cells subgroup.Methods:The expression of CD44, EpCAM, CD 166 and CD271 in 10 cases of normal mucosa, 20 cases of adenoma and 60 cases of gastric adenocarcinoma were detected by SP (streptavidin HRP) immunohistochemical staining in successive section. The locations of EpCAM and CD44 double positive cells were detected by HE staining, SP (streptavidin HRP) immunohistochemical staining and double immunohisto-chemical staining. The morphologic characteristics of EpCAM and CD44 double positive cells were observed. The expression of CD44, EpCAM, CD 166 and CD271 in human gastric adenocarcinoma cell line BGC-823 were detected by SP (streptavidin HRP) immunohistochemical staining and the ratio and the morphologic characteristics of positive cell subgroup were detected. Results:1. The levels of expression of CD44 and EpCAM in normal gastric mucosa, adenoma, adenocarcinoma were increased in proper order (rs=0.646, p=0.000; rs=0.495, p=0.000). The levels of expression of CD44 in gastric adenocarcinoma was related with gender, lymph node metastasis and depth of infiltration. There was a positive correlation between the expressions of CD44 and pathological grade (rs=0.297, p=0.021). The levels of expression of EpCAM was related with gender, lymph node metastasis and depth of infiltration.The expression of CD44 was positively correlated to that of EpCAM in gastric adenocarcinoma (rs=0.389,p=0.002)2. The expression of CD166 in normal gastric mucosa and adenoma was significant higher than that in adenocarcinoma. The expression level of CD166 was positively correlated to the tumor differentiation and related with depth of infiltration (rs=0.597, p=0.000) (p=0.000). The levels of expression of CD271 in adenocarcinoma was significantly higher than that in nomal gastric mucosa and adenoma. The levels of expression of CD271 was related with lymph node metastasis (p=0.000) and depth of infiltration (p=0.000)3. There was no EpCAM and CD44 double positive cells in nomal gastric mucosa and just a few EpCAM and CD44 double positive cells in gastric adenoma were detected. More EpCAM and CD44 double positive cells were detected in gastric adenocarcinom. The number of double positive cells accounted for 1% to 10% of all tumor cells, and the cells scattered or distributed focally surrounded the tumor nest or located in the depth. The double positive cells were small, round and nucleus was anachromasised. The quantity of double-positive cells in gastric adenocarcinoma with or without lymph node metastasis was remarkbly different and the former was higher. The more depth infiltrated, the more quantity of double-positive cells observed.4. The expression of CD166,CD271 and EpCAM in cell line BGC-823 was negative. The positive rate of CD44 in cell line BGC-823 was 52.90±6.37%. The positive cells were small, round and their nucleus was anachromasised. Conclusion:1. CD44 and EpCAM were identified as stable gastric adenocarcinoma stem cell maker. The detection of the two markers will be applicable guidance for the diagnosis of adenocarcinoma metastasis, depth of infiltration of the patients and clinical therapy.2. There were some expression of bone marrow-derived mesenchymal stem cells maker CD166 in gastric mucosa, adenoma and adenocarcinoma, but the CD271 was just showed lower expression in adenocarcinoma. The distribution of positive cells in gastric mucosa was irregular. CD 166 and CD271 was not the specific cancer stem cell maker of gastric adenocarcinoma.3. The distribution and quantity of gastric adenocarcinoma stem cell in gastric adenocarcinoma were closely related with metastasis and infiltration of gastric adenocarcinoma. To evaluate the number, distribution and morphology of cancer stem cell and the relationship between pathological parameters and gastric adenocarcinoma stem cell will provide guidance for the diagnosis, treatment and clinical prognosis of gastric adenocarcinoma.4. CD166, CD271 and EpCAM were not the stem cell maker of gastric cancer cell line BGC-823, but CD44 was an available stem cell maker. |
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