| The purpose:Isolated SGC-7901 gastric cancer cell line containing CD44+ c-ells,identified isolated CD44+cells whether gastric stem cells, And gastric ca-ncer stem cells and gastric carcinoma, metastasis, recurrence relations between.Methods:the gastric cancer cell line SGC-7901 were cultured from the purch-ase, to be amplified to a certain amount of control group by flow cytometry were selected to the experimental group containing CD44+cells with CD44+ cells, respectively. The reserve. Two groups of cells to culture one month, Th-e first, Third, Fifth, Seventh, Ninth and Fifteenth by MTT colorimetric meth-od for the determination of various cells proliferation, the absorbency and the growth days are longitudinal and transverse drawing growth curve, and by c-omparing the 2 groups of cells proliferation rate curve. The use of statistical methods the proliferation of cells in the two groups were analyzed.The CD44+ cell count of the separation, were selected for cell number is 1×102, 1×103, 1×104 three gro-ups, named as A, B, C three groups. NOD/SCID mice wer-e randomly divided into 3 groups to buy, the number of a, B, C three group s, respectively, the A, B, C three groups of cells into three groups of NOD/ SCID mice the mice were observed for 8 weeks, subcutaneous and whether t-here is tumor morphology and growth of mice subcutaneous tumor in three g r-oups compared with the tumor; tumor mice were sacrificed to cut the subc-u taneous tumor biopsy, pathological sections and compared the similarities and differences of gastric cancer.Results:in the experimental group compared with the control group, with the growth of CD44+cells did not contain CD44+cell growth significantly;the dif-ference was statistically significant between the experiment group and the co-ntrol group (t=2.920, P<0.05); 1×103 tumor formation rate was significantly hi-gher than that of the other two groups were injected into NOD/SCID mice cells, 1×103 tumor formation time was earlier in the other two groups were in jected into NOD/SCID mice cells, the difference was statistically significant (t=2.353, P<0.05).Conclusion:The gastric cancer cell populations with selected with CD44+ by flow cytometry cell ratio of 5%, is relatively low; CD44+cells can grow int-o tumors in the ball under the condition of in vitro culture, and can carry o-ut continuous passage; detection of CD44+ cell viability by MTT assay, the s-elected CD44+cells in 1 days to 3 days and 5 days to 7 days in vitro ampl-ification conditions were the most significant growth; CD44+ cells are highly t-umorigenic cells in appropriate circumstances, and in a few not tumorigenic, large dose of tumorigenic ability is low or tumorigenic cycle longer; gastric c-arcinoma containing CD44+ cells have some characteristics of embryonic ste-m cells, and can be isolated and cultured in vitro, which have the ability of self-renewal, tumorigenicity, is considered to be the gastric cancer stem cells. CD44+ is a cell surface marker of gastric cancer, can be used as a molecular marker for the identification of gastric cancer stem cells. |
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