Study On The Role Of Human Cytomegalovirus UL146 Gene-α Chemokine In Cytomegalovirus Infection

ObjectiveTo investigate the polymorphism of human cytomegalovirus (HCMV) UL146 gene in clinical strains, and to find the clinical diagnosis,therapeutic value of gene and gene's changes.MethodsThe UL146 gene regions of clinical strains in which positive HCMV-DNA in urine was detected by using quantitative polymerase chain reaction(QPCR) were amplified by polymerase chain reaction(PCR).Positive samples of PCR amplification were sequenced and analysed.ResultsHigh variability was found in UL146 gene among 28 HCMV clinical strains. According to the phylogenetic analysis,all sequences of UL146 in clinical strains could be divided into three types, four subtypes.G1 group of amino acid sequence homology with the Toledo strain was 96.6%-98.3%,G2 group,10.3%-22.2%,G3 group,12.8%-13.7%.ELRCXC chemokine function area was highly conserved in all sequences.ConclusionHCMV-UL146 genes show a high degree of polymorphism,and its encoded chemokine ELRCXC functional area is highly conserved. HCMV-UL146 gene's polymorphism didn't show obvious relationship with different clinical symptoms of HCMV infection.