| Matrix metalloproteinase (MMP) is one of the mostly important protease of cleaving extracellular matrix. It rarely express in normal tissues. MMP-9 mainly been synthesized and secreted by neutrophils and macrophages, its mRNA and protein can be detected in cortex, hippocampus and striatum, but the abundance is extremely low in normal state. Its expression increased during a variety of pathological processes of the body, such as inflammation, angiogenesis, tumor invasion and metastasis. MMP-9 may participate in the secondary injury development of ischemic stroke through the degradation of components of cerebrovascular basement membrane, such as gelatin, laminin and fibronectin.Objective: In this study, in order to exploring the mechanism of sodiumβ-aescin against the cerebral ischemia injury, the expression of MMP-9 is detected in the rat with middle cerebral artery occlusion.Methods: 60 Wistar rats were randomly divided into control group, model group, low dose treatment group and high dose treatment group. Using a modified middle cerebral artery second suture method from Zea-Longa, the left middle cerebral artery occlusion of rat was carried out, sodiumβ-aescin was administrated at 6 hours, 24 hours, 48 hours and 96 hours after infarction. And the expression of MMP-9 and its mRNA was detected with immunohistochemistry and in situ hybrization methods.Results:Compared with the control rats, the expression of MMP-9 and its mRNA in brain tissue of model group increased. And compared with model group, the MMP-9 and its mRNA expression decreased slightly in the group of low dose treatment, decreased more significantly in group of high-dose treatment (P <0.05).Conclusions: MMP-9 expression increases in a manner of time-dependent after middle cerebral artery infarction. Sodiumβ-aescin can decrease the expression of MMP-9 and its mRNA, inhibit the development of brain edema, protect the brain from injury.
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