Studies On Chemical Components And Antioxidant Activities Of Corydalis Ochotensis Turcz

Corydalis Ochotensis Turcz is the dry herb of a Papaveraceae perennial herbaceousPlant,grown in the northeast of china.The raw material are used in Chinese folk herbal medicine for treatment of bleeding,ache,tuberculosis,tumor and edema.View from the current study,other medicines of the Corydalis have very significant biological activity in the cardiovascular system, nervous system and so,but for the Corydalis Ochotensis Turcz is still blank in China,so research and exploration its chemical composition and biological activity has great practical significance.In this paper,the chemical compositions and antioxidant activities of Corydalis Ochotensis Turcz were studied. Mainly include the following aspects of the research work:1. After reading a lot of literatures based on the overview of Corydalis plant chemical compositions, biological activities and the research progress of Corydalis Ochotensis Turcz and method for determination of alkaloid content.2. First pre-test the chemical composition of Corydalis Ochotensis Turcz to provide a theoretical basis for the next extraction and separation. The results showed that Corydalis Ochotensis Turcz contains alkaloids, saponins, organic acids, sterols, sugars and glycosides, phenolic compounds, cardiac glycosides. Alkaloid is the main chemical compositions of Corydalis Ochotensis Turcz and content is the highest.3. The dry herb of Corydalis Ochotensis Turcz with 70% ethanol soaking,then acidification and organic solvent extraction,has been part of petroleum ether extract, chloroform extract and n-butanol extract. Use of column chromatography, recrystallization, TLC and other methods chloroform extract of the Corydalis Ochotensis Turcz were isolated and two compounds were isolated. According to physical and chemical properties,chemical methods,1HNMR,13CNMR,HMBC and their structures identified,namely capnoidine (I) and protopine (II). After sincefinder, CA and other database search to identify compound I for the first time I get from the Corydalis Ochotensis Turcz.4. Using the GC-MS the petroleum ether extracts of Corydalis Ochotensis Turcz were studied and 29 kinds of chemical constituents isolated. In NIST1998. L database support and consult relevant literature material identified of 16 kinds of components. Combining normalization method to calculate the relative contents of them. From the results,the main compounds are long-chain diolefine, alkenes, esters, acids. The highest content of long-chain diolefine, the carbon atoms is generally between 14 to 28, mainly have pentacosane (7.02%), octacosane (6.97%),Tetracosane(6.30%)and the total content of 38.74%. In addition, tricosane, hexacosane, docosane and heneicosane very high content in the total compounds, respectively 5.65%,4.69%,3.57%,2.22%. Hexadecanoic acid(14.67%),lipids and alkenes content is also high. Can be seen that hexadecanoic acid content is the highest. According to the literature, hexadecanoic acid has anti-tumor activity, this study for the development of antitumor activity provided the basis.5. This is the first established the content determination of chloroform extract of Corydalis Ochotensis Turcz by acid-dye colorimetry.First of all, the determination conditions were screened to determine the best conditions for determination,the Specific means is:With berberine hydrochloride as the control article, precision to measure a certain volume of berberine hydrochloride and test solution in evaporating dish in water bath. Residue with a pH3.6 sodium citrate buffer solution and transferred to a separatory funnel. Bromocresol green solution by adding 7ml, shaking 5min, adding chloroform 10ml, shaking 5min, stand for 1h, chloroform layers obtained by adding 0.5g of sodium sulfate anhydrous, standing for 0.5h, filtered, determination of absorbance at 415nm. Secondly, do the precision experiments,stability experiments,recovery experiments and a series of methodology experiments. Alkaloid content was determined, Its content is 70.9%. The method is simple, reliable, and can be used as content determination method of chloroform extract.6. Studied the Antioxidant activity of the chloroform extract,reducing power and remove ability of the DPPH radical,hydroxyl radical,superoxide anion radical capacity were investigated. The results show that,Chloroform extract has the ability to reducing power and concentration had positive correlation. Chloroform extract can be different levels of three free radical scavenging,remove ability of hydroxyl radicals is weak and remove ability of DPPH radicals and superoxide anion radical is strong.