The Content Determination Of Herba Solani Lyrati

Herba Solani Lyrati is a kind of dry herb of Solanum lyratum Thunb.It was recorded in The Shennong Herb-Root Classic,Pharmacopoeia of the People's Republic of China(1977 Edition) at first,and then Chinese medicine standard of Bei Jing,Chinese medicine standard of Guang Xi(1996 Edition),Chinese medicine standard of Gui Zhou,Chinese medicine standard of He Nan(1993 Edition) have recorded as well.Herba Solani Lyrati has the functions of reducing fever, detoxification,dispersing pathogenic wind,removing dampness to reduce phlegm.Herba Solani Lyrati is wildly distributed all over the country,the provinces are in the south of Yellow River almost,and China is rich in natural resources.Through the modern pharmacological research,Bai Ying is anti-inflammatory,antifungal and has the effects of antitumorigenesis.However,the research is not deep and systematic enough nowadays.Pharmacopoeia of the People's Republic of China(1977 Edition) and Chinese medicine standard of every places just recorded the character identification with the item of Bai Ying,there is no record about its safety and quality.This essay intends to do the pharmacognostics research of Bai Ying,then we can constitute a preliminary quality standard of Bai Ying to ensure its safe,effective, steady and controllable.Through collecting Chinese 8 provinces,21 producing places,26 samples of Bai Ying crude drugs,checking and extractive determination,including determination of moisture,ash content test,acid non-soluble of ash,water-solubility extract and spirit soluble extract,which lay the foundation to make an initial draft of Bai Ying's quality standard.It is the first time to have a prior system testing of chemical composition of Bai Ying.This article is first time to set'up a preliminary and best development system of TLC(thin layer chromatography) for solasodine and rutin within Bai Ying. Results state that:benzene-methanol(10:2) is the best mobile phase to develop solasodine.The spot which is used the developer is clear,the color is obvious,Rf is suitable;ethyl acetate- acetone- methanoic acid-water(8:1:1:1) is the best developer for rutin.A combination of laboratory result of tset tube and TLC,we can ascertain that the kinds of chemical ingredient in Bai Ying,they are phenolic constituents,alkaloid, flavone,organic acid,aminophenol,protein,sterine,triterpene and so on, without anthraquinone or cardiac glycoside.It is also the first time to do the research about the safety evaluation and pharmacodynamics of Bai Ying crude drug.Through acute toxic experiment and the max dosage to a mouse experiment to ascertain the safe extent of Bai Ying clinical dosage;through the mouse's auricular swelling and acetic acid writhing test.we can know Herba Solani Lyrati has the the functions of reducing fever, detoxification,dispersing pathogenic wind,removing dampness to reduce phlegm,it is the same to material medica of past dynasties and literature recordation.Modern application of Herba Solani Lyrati extracts its alkaloid constituents as effective ingredient,so this essay uses UV to determination the total alkaloid in Bai Ying,provides reference for perfect the quality standard draft of Bai Ying.