| Background:Programmed cell death 4(PDCD4) is a tumor-suppressor gene which is correlated with cell death and tumor suppression. PDCD4 is significantly lowly expressed in a number of carcinomas such as lung, ovarian, cervical, breast, and gastric cancer. More and more reports about PDCD4 function and regulatory mechanism have been published recently. PDCD4 protein maybe valuable for the diagnosis and treatment of tumors.However, little is known about expressions of PDCD4 mRNA and protein in renal cancer.Objective:1 To detect expressions of PDCD4 mRNA and protein in clear cell renal carcinoma (ccRCC).2 To study the correlation of PDCD4 with clinical and pathological parameters of clear cell renal carcinoma.Methods:Semi-quantitive reversed-transcription polymerase chain reaction (semi-quantitive RT-PCR) and immunohistochemistry were applied to evaluate the expressions of PDCD4 mRNA and protein in clear cell renal carcinoma and their adjacent non-tumor renal tissues. (β-actin served as a quantitative standard marker).Results:PDCD4 mRNA was detected both in the normal tissues and ccRCC, the amount of PDCD4 mRNA in normal tissues was 0.83±0.27 while that in cancer tissues was 0.62±0.24 (pdcd4/β-actin).The former showed higher PDCD4 mRNA expression than the latter (t=5.664 P<0.05), the positive rate of PDCD4 protein (37.5% 15/40) in ccRCC was significantly lower than their adjacent non-tumor renal tissues (90% 18/20).Low grade (Fuhrman grades 1 and 2) ccRCC showed higher PDCD4 protein expression than high grade ones (P<0.05). The positive rate of PDCD4 protein in I-II grade ccRCC was 38.7%(12/31)while that inⅢ～ⅣccRCC was 33.3%(3/9).The difference was not significant(P>0.05).Conclusions:1 PDCD4 gene is closely associated with carcinogenesis and development of renal cancer.2 PDCD4 protein is correlated with clinical and pathological parameters of clear cell renal carcinoma, and would be valuable for the diagnosis and treatment of renal cell carcinoma.
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