| Objects1.To evaluate the clinical effect and security of Xiezhuo Chubi decoction on Hyperuricemic patients, by observing the changes of indexes, including uric acid, clinical symptoms and indicators of safety.2. To investigate the effects of Xiezhuo Chubi decoction on uric acid level in Model of Hyperuricemic Mice, that to prove its effectiveness.3. To investigate the influence of Xiezhuo Chubi decoction on URAT1 gene in the kidney of Hyperuricemic Mice, that to search for the mechanism of reducing uric acid.Methods1.99 patients of Hyperuricemia were randomly divided into Xiezhuo Chubi decoction group, Benzbromarone group and Control group. The patients in Xiezhuo Chubi decoction group were treated by concentrated granules of Xiezhuo Chubi decoction. The patients in Benzbromarone group were treated by Benzbromarone tablets. The patients in Control group were treated without drugs. There were 20 days for a course of treatment. The clinical symptoms, physical signs and laboratory data before and after treatment were noted and compared.2.60 Kunming male mice of SPF grade were randomly divided into High-dose Xiezhuo Chubi decoction group, Medium-dose Xiezhuo Chubi decoction group, Low-dose Xiezhuo Chubi decoction group, Benzbromarone group, Model group and Control group. All mice were established with yeast method combined with uricase inhibition method to build Hyperuricemia model except Control group, and the corresponding drugs were administrated at the 7th day. On the 22nd day, the eyeballs of mice were removed and the blood was obtained to detect uric acid levels. Simultaneously, the kidneys were removed to detect mRNA expressions of URAT1 with Fluorescence quantitative PCR and RT-PCR.Results1. The significant decrease of the degree of uric acid was found in the three groups of Hyperuricemic patients after treatment (P<0.05). The total effective rate was 85.71% in Xiezhuo Chubi decoction,92.86% in Benzbromarone group, and 23.33% in Control group. There was no statistically significant difference between Xiezhuo Chubi decoction and Benzbromarone group (P> 0.0167). The effect in these two groups was similar, and was better than that in Control group (P<0.0167).2.The clinical symptoms of patients in Xiezhuo Chubi decoction group, included phlegm, lassitude, heaviness of limbs, dark urine and loose stool were improved significantly, and the urinary volume was increased after treatment (P<0.05).3. The laboratory data of patients in Xiezhuo Chubi decoction group, included blood routine, liver function, renal function and urine routine, were no significant difference after treatment (P>0.05).4. Imposed by different factors to each group, oral administration of Xiezhuo Chubi decoction could significantly reduce the uric acid in mice in a dose-dependent manner, and it was significant difference than that in Model group (P<0.05). The effects of High-and Medium-dose Xiezhuo Chubi decoction group were similar with Benzbromarone group (P>0.05), while the effect of Low-dose Xiezhuo Chubi decoction group was weaker than Benzbromarone (P< 0.05).5. Detected by Fluorescence quantitative PCR and RT-PCR, the mRNA expressions of URAT1 in the kidney of Hyperuricemic Mice were decreased significantly in High-, Medium-, Low-dose Xiezhuo Chubi decoction group and Benzbromarone group than that in Model group (P<0.05), but the effects of inhibition of Xiezhuo Chubi decoction were weaker than Benzbromarone (P< 0.05).Conclusions1.Xiezhuo Chubi decoction could reduce the uric acid and improve a part of clinical symptoms effectively in Hyperuricemic patients, while it is safety. 2. Xiezhuo Chubi decoction could reduce the uric acid effectively in Hyperuricemic mice. Its mechanism of reducing uric acid might be the action of inhibition to the mRNA expressions of URATl in the kidney of Hyperuricemic Mice.
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