The Significance And Changes Of CD44 In The Graft Of Acute Rejection Rat Renal Transplantation Model

Objective:This study want to investigate the Preventive effects of the extract of Erigeron on acute renal allograft rejection and to explore its mechanism.To assess relationship between the renal graft expression of CD44 and the graft acute rejection.Methods:1.20 pairs of male inbred strain Wistar rats were both the donors and receptors who were forbidden to food but accessed to water before the operation. The excision of the donor's kidney was done after low-temperature perfusion in situ under the anesthesia by peritoneal injection of 3% sodium pentobarbital by applying microsurgical techniques. Recipients kidney was removed also by applying microsurgical techniques. The both kidneys of the donor was transferred immediately to the recipient. The transferred material included the ureter and the aorta and the inferior rena cava. The aorta of the donor was sutured to the recipients'end-to-side. The inferior rena cava were sutured with the aid of epidural anesthesia channel. The ureter with bladder valve of the donor was sutured to the recipient.2. Male inbred strain Wistar rats (n=60) and out-bred strain SD rats (n=30) were donors and receptors. Control group was normal male Wistar rats;syngeneic renal transplantation control group(sTX)was the Wistar rats acted as both donors and recipients;allogenenic renal transplantation(aTX)was the SD and Wistar rats acted as donors and recipients respectively. Immunodepression group (aTX+CsA) was aTX with the administration of ciclosporin A. The semi-quantity scores of AR were compared between groups of 5 and 7 d after TX, by Banff acute rejection indexes, Immunohistochemical staining was used to investigate the expression of CD44 on untransplanted kidneys and grafts. Serum creatinine were assayed at the same time. And the correlations were analyzed.Results:1. graft turned cherry immediately after reperfusion, the engorged renal artery impulse was good and urine was formed 3-5 min after blood current rebuilt.Total operation time was (130±20)min; heat ischemia time of the grafts did not exceed 10 sec and cold ischemia time did not exceed 50 min.The achievement ratio of transplantation exceeded 82%.2. Results showed that four group 5 and 7 d after TX was significance in the semi-quantity scores of AR (H=16.63,p=0.001),(H=17.37,p=0.001);.compared with the sTX group and aTX+CsA,5 and 7 d after TX, the semi-quantity scores of AR in aTX group were increased significantly(p<0.01). the expression of CD44 in TX renal in aTX group were increased significantly with the control group and the aTX+CsA(p<0.05). The SCr in TX renal in aTX group were increased significantly with the control group and the aTX+CsA (p<0.05). CD44 expression increased significantly in the acute rejection group compared with the non-rejection groups (P<0.05). Renal grafts CD44 expression correlated with acute rejection and SCr (r=0.431,P<0.01,n=36)(r=0.458,P<0.01,n=36), whereas in rats developing acute rejection it did not correlate with Banff scores (r=0.403,P>0.05,n=36).Conclusions:1. In conclusion,the kidneys with the Wistar rats acting as both donors and receipts could play the role of control to renal transplantation acute rejection rat models because of the lagging AR and low degree of AR;so the kidneys with SD and Wistar rats acting as donors and receipts respectively were the renal transplantation acute rejection rat models.2. CD44 plays an important role in the pathogenesis of acut allograft rejection. Further studies of CD44 may help to promote the development of new approaches for controlling and preventing graft acute rejection.