The Study On Expression And Relationship Of HLA-G,IL-10in Urothelial Carcinoma After Renal Transplantation

Objective:The purpose of this study was to examine the expression of human leukocyte antigen-G(HLA-G) and its relation with interleukin-10(IL-10) production in patients with urothelial carcinoma after renal transplantation.Methods:This study included28patients with histopathologically proven urothelial carcinoma,14of whom had undergone renal transplantation, and14others who had not and served as a control group1.5normal mucous membranes of urinary bladder were selected as a control group2. HLA-G and IL-10expression in urothelial carcinoma was determined using immunohistochemistry method. SPSS18.0statistical software was applied to anlysis the data and statistical significance was defined as P values less than0.05.Results:Among the transplantation group, HLA-G and IL-10protein expression were detected in78.57%(11/14) and78.57%(11/14); Among the control group1, HLA-G and IL-10protein expression were detected in28.57%(4/14) and78.57%(11/14); Among the control group2, IL-10protein expression were detected in20%(1/5), However, immunohistochemistry assay showed no HLA-G protein expression in5normal mucous membranes of urinary bladder. The expression of HLA-G between transplantion group and control group1showing significant difference(P<0.05). The HLA-G expression did not show any significant correlation with IL-10expresion no matter at the level of protein.Conclusion:The results indicate that the expression of HLA-G and IL-10might play an important role in progression of urothelial carcinoma. HLA-G expression is associated with progression of post transplantation urothelial carcinoma,it might be used as a prognosis marker to estimate the patients’levl of the immunosuppression who undergone renal transplantation.