The Expression And Clinical Significance Of Annexin A10 In Human Gastric Cancer

ObjectiveThe Annexin A10 (ANXA10) gene is located on chromosome 4q33, as a novel member of annexins (ANXs) superfamily, the biological function of which has unknown. It may have the potential to inhibit the tumor growth, our previous study located the loss areas of chromosome 4q near the locus of ANXA10. To investigate the association with ANXA10 gene and gastric cancer, we conducted immunohistochemistry to research the protein expression of ANXA10 in gastric cancer and corresponding adjacent normal gastric tissue, and we also evaluated the expression level of ANXA10 in relation to clinicopathological characteristics and prognosis.Materials and MethodsAll 145 tissue samples were obtained from the patients with primary gastric cancer underwent curative gastrectomy at the Department of Oncology, First Affiliated Hospital of China Medical University from January 2003 to September 2003. Before surgery, all patients had not received any chemotherapy or radiotherapy. All specimens were fixed with 10%formalin, embedded in paraffin, cut into multiple 4μm sections. The sections were routinely stained with hematoxylin and eosin for histopathologic diagnosis and remaining sections were used for immunohistological analysis. Two-tailed chi-square tests, Kaplan-Meier estimator, log-rank test were employed to analysis the association with clinicopathological characteristics, prognosis and expression level of ANXA10. ResultsANXA10 was immunolocalized to the nuclear and cytoplasm. The positively expression rate of ANXA10 in gastric cancer tissues was 49.7%, significantly lower than that in corresponding adjacent normal gastric tissues(98.6%)(p<0.001). Downregulated expression of ANXA10 was highly correlated with tumor location (p=0.047), depth of invasion (p=0.008), lymph nodes metastasis (p=0.006), pTNM stage (p=0.008). However, there was no significant difference between downregulated expression of ANXA10 and sex, age, tumor size, Borrmann type, histologic grade. The prognosis of patients with tumors expressing ANXA10 protein was significantly different with which of ANXA10-negative patients.Conclusion1,ANXA10 was downregulated expression in gastric cancer.2,Downregulation expression of ANXA10 may play an important role in the tumor progression.3,ANXA10 may serve as a prognostic marker in gastric cancer.