Expression Of PTEN,Her-2/neu And PKB In Cholangiocarcinoma And Its Significance

ObjectiveTo explore the expression of PTEN,Her-2 and PKB in cholangiocarcinoma and to investigate the role of their alteration in tumorigenesis and progression of cholangiocarcinoma.MethodsWe detected the PTEN,Her-2 and PKB in 80 cholangiocarcinoma and 24 normal bile duct tissues by the immunohistochemistry SP method.ResultsPTEN expression in cholangiocarcinoma was significantly lower than normal bile duct tissues (X2= 11.33, P=0.001), HER-2/neu expression was significantly higher than that in normal bile duct tissues (X2= 13.16, P=0.000), and PKB expression was significantly higher than that in normal bile duct tissues (X2= 6.41, P=0.01). PTEN and HER-2/neu protein expression was negatively correlated (r=-0.456, P= 0.000);PTEN and PKB protein expression was negatively correlated (r=-0.355, P= 0.001); HER-2/neu and PKB protein expression was positively correlated (r= 0.374, P= 0.001).ConclusionsLoss of PTEN expression and HER-2/neu over-expression may lead to loss of control of cell proliferation, and are closely related with the occurrence,development of cholangiocarcinoma, both were associated with the PKB, suggesting that they may pass the PI3K-PKB signal transduction pathway involved in the occurrence and development of cholangiocarcinoma.