Effect Of Chrysalis Oil On Blood Glucose And Oxidative Stress In Diabetic Rats

ObjectiveTo investigate the effect of chrysalis oil on blood glucose and oxidative stress in diabetic rats caused by Streptozotocin(STZ).Methods1.Chrysalis oil was extracted by combination of microwave extraction technology and urea adduction fractionation.Chrysalis oil riches in unsaturated fatty acids such asα-linolenic acid,linoleic acid.And the levels of unsaturated fatty acids were higher than pupa oil which was extracted by traditional methods.2.60 male SD rats were divided into 6 groups according to blood glucose: normal group,diabetic control group,high,medium,low dose therapeutic groups,and positive control group.Food and fluid intake were monitored everyday,blood glucose and weight were tested at each weekend,at the end of experiment,Glycosylated serum protein(GSP) was tested,the effect of chrysalis oil on the content of hepatic glycogen and Malondialdehyde(MDA),Reduced glutathione(GSH),Total antioxidant capacity(T-AOC) in liver and pancreas were observed.Results ①Diabetic symptoms were ameliorated and blood glucose and GSP were all lowered in therapeutic groups compared with diabetic control group,whereas,high dose group had the same effect as positive control group.②The content of hepatic glycogen was obviously decreased in diabetic control group compared with normal group,whereas,the content of hepatic glycogen was increased in high and medium dose therapeutic groups compared with diabetic control group.③The content of MDA was obviously increased and the activities of GSH and T-AOC were decreased in diabetic control group compared with normal group. However,in diabetic groups treated with chrysalis oil,the activity of GSH and T-AOC were significantly increased and the content of MDA decreased compared with diabetic control group.ConclusionChrysalis oil could decrease blood glucose in a dose-and-effect relationship and improve antioxidant capacity.